The portrait of Lisa di Anton Maria di Noldo Gherardini, third wife of Francesco di Bartolommeo de Zenobi del Giocondo. She is seated in a chair on which her left arm rests, her right hand superposed on the left. She is turned three-quarters to her right. Her hair, divided in the centre and seen under a transparent veil, falls in curls on her shoulders; her dark almond-shaped eyes look out at the spectator; the mouth is smiling. She wears a dark-green dress with golden-brown sleeves; a dark cloak is draped over her shoulders. The background is formed by a mountainous landscape full of incident.

Painted in tempera on panel, and restored in oil.

2 ft. 6½ in. × 1 ft. 9 in. (0·79 × 0·53.)