CONTENTS

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Historical Introduction—Spontaneous Generation—Causation of Disease—Putrefaction and Fermentation—Study of Forms—Chronological Table 17
CHAPTER I.
Position of Bacteria—Relationships to AlgÆ—Yeasts—Molds—Protozoa 37
PART I.
MORPHOLOGY.
CHAPTER II.
Cell Structures—Cell Wall—Protoplasm—Plasmolysis—Plasmoptysis—Nucleus—Vacuoles—Capsules—Metachromatic Granules—Flagella—Spores 41
CHAPTER III.
Cell Forms—Coccus—Bacillus—Spirillum—Involution Forms 52
CHAPTER IV.
Cell Groupings 55
CHAPTER V.
Classification—Migula’s—Society of American Bacteriologists’—Key to the Latter 59
PART II.
PHYSIOLOGY.
CHAPTER VI.
Occurrence—General Conditions for Growth—Moisture—Temperature—Light—Oxygen—Osmotic Pressure—Electricity—X-rays and Radium Emanations—Pressure—Mechanical Vibration 71
CHAPTER VII.
Chemical Environment—Reaction of Medium—Chemical Composition 81
CHAPTER VIII.
Chemical Environment (Continued)—General Food Relationships—Metabolism of Elements 86
CHAPTER IX.
Physiological Activities—Fermentation of Carbohydrates—Splitting of Fats 93
CHAPTER X.
Physiological Activities (Continued)—Putrefaction of Proteins—Cycles of Nitrogen, Carbon, Sulphur, Phosphorus 102
CHAPTER XI.
Physiological Activities (Continued)—Production of Acids, Gases, Esters, Alcohols, Aldehydes, Aromatic Compounds—Phosphorescence—Chromogenesis—Reduction—Oxidation—Production of Heat—Absorption of Free Nitrogen—Nitrogen Nutrition of Green Plants 110
CHAPTER XII.
Physiological Activities (Continued)—Production of Enzymes—Discussion on Enzymes—Toxins—Causation of Disease 121
CHAPTER XIII.
Disinfection—Sterilization—Disinfectants—Physical Agents—Pasteurization 130
CHAPTER XIV.
Disinfection and Sterilization (Continued)—Chemical Agents—Anilin Dyes 156
CHAPTER XV.
Disinfection and Sterilization (Continued)—Choice of Agent—Standardization of Disinfectants—Phenol Coefficient—Practical Sterilization and Disinfection 164
PART III.
THE STUDY OF BACTERIA.
CHAPTER XVI.
Culture Media—Broth, Milk, Gelatin, Agar, Potatoes, Blood Serum—Standardization of Media—H-ion Concentration Method—Synthetic Media 171
CHAPTER XVII.
Methods of Using Culture Media—Culture Tubes—Plates—AnaËrobic Cultures—Vignal Tubes—Fermentation Tubes—Deep Culture Tubes—Novy Jars—Inoculation of Culture Media 184

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