CONTENTS.

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Sylvester Marsh
PART I.
Introduction 11
On Cutting Unprepared Vegetable Tissues 12
On Cutting Unprepared Animal Tissues 13
Preparation of Vegetable Tissues 15
Preparation of Animal Tissues 16
Special Methods of Hardening 19
Cutting Hardened Tissues by Hand 19
Microtome 20
Æther Microtome 24
Section-Knife 24
Imbedding in Paraffine for Microtome 26
Employment of Microtome 29
Staining Agents 31
Carmine Staining 32
Mounting Media 36
Mounting in Glycerine 38
Uses of Freezing Microtome 42
Employment of Freezing Microtome 43
Logwood Staining 47
Absolute Alcohol 50
Clove Oil 51
Canada Balsam 51
Mounting in Balsam 53
Finishing the Slide 55
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