AbbÉ's condenser, 7
Abbott's stain for spores, 107
Aberration, chromatic, 56
spherical, 55
Absolute alcohol as a fixative, 82
as an antiseptic, 27
Absorbent paper for drying cover-slips, 69
A. C. E. mixture, 345
Acetic acid for clearing films, 82
Achromatic condenser, 54
Acid hÆmatin, 96
production, analysis table, 283
by bacteria, 145
investigation of, 280
qualitative examination, 283, 284
quantitative examination, 280
Acid-fast bacilli in tissues, to stain, 124
Action of various gases on bacteria, 295
Active immunisation, illustrative example, 322
Adjustable water bath, 299
Aerobic cultures, 221
Aerogenic bacteria, 131
Aesculin agar, 204
Agar gelatine (guarniari), 194
methods of preparation, 167
surface plates, 232
Agar-agar, preparation of, 167
Agglutination reaction, macroscopical, 386
microscopical, 385
Agglutinin, 381
Air, analysis of, 468
filter, 40
pump, Geryk, 43
Albumin solution, Mayer's, 120
Alcohol production, test for, 285
Alkaline pyro, 239
Alum carmine, 96
Ammonia production test for, 285
Amphitrichous bacteria, 136
Anaerobic cultures, 236
Botkin's method, 243
Buchner's method, 238
Bulloch's method, 245
Hesse's method, 237
McLeod's method, 240
media, 180
Novy's method, 244
Anaerobic cultures, Roux's biological method, 237
physical method, 237
vacuum method, 238
Wright's method, 239
AnÆsthetics, 345
Analysis of air, apparatus for, 469
method of, 468
qualitative bacteriological, 470
quantitative bacteriological, 468
of butter, qualitative bacteriological, 458
quantitative bacteriological, 457
of cream, qualitative bacteriological, 458
quantitative bacteriological, 457
of fish, 460
of ice cream, qualitative bacteriological, 457
of meat, apparatus for, 460
method of, 460
qualitative bacteriological, 462
of milk, apparatus for, 444
collection of samples, 441
method of, 441
qualitative bacteriological, 446.
quantitative bacteriological, 444
of oysters, 463
of sewage, qualitative bacteriological, 467
quantitative bacteriological, 466
of shellfish, 463
of soil, apparatus for, 473
collection of samples, 471
method of, 470
qualitative bacteriological, 476
quantitative bacteriological, 473
of water, apparatus for, 420, 427
collection of samples, 416
method of, 416
qualitative bacteriological, 426
Analysis of water, quantitative bacteriological, 420
Aniline dyes, 83
Gentian violet, 95
water, to prepare, 108
Animal tissue media (Frugoni), 210
Animals, natural infections of, 337
Antiformin method for B. tuberculosis, 502
Antigen, definition of, 324
Antiseptics, 27
action of, 310
Apparent filth in milk, 450
Arnold's steam steriliser, 34
Arthrogenous spores, 138
Ascitic bouillon, 210
fluid agar (Wassermann), 213
AscomycetÆ, 128
Ascopores, 129
Asparagin Media (Frankel and Voges), 183
(Uschinsky), 183
Aspergillus, 127
Atmospheric conditions, 295
Attenuating the virulence of organisms, 321
Autoclave, 37
to use, 37
Automatic pipettes, 13
Autopsies, 396
Autopsy, card index for, 402
Bacilli, morphology of, 132
Bacillus anthracis in soil, 477
in water, 440
coli in water, detection of, 429
diphtheriÆ in milk, 452
enteritidis in water, 437
sporogenes in milk, 452
in water, 438
oedematis maligni in soil, 477
tetani in soil, 477
in water, 441
tuberculosis in milk, 453
antiformin method, 502
typhosus in water, 441
Bacteria, anatomy of, 134
classification of, 131
grouping of, for study, 410
in tissues, demonstration of, 114
influence of environment on, 142
metabolic products of, 143
methods of identification, 259
microscopical examination of, stained, 81
unstained, 74
physiology of, 136
Bacteria, simple stains for, 90
Bacterial emulsion, preparation of, 389
enzymes, 144, 277
ferments, 144
food stuffs, 142
toxins, 144
Bacteriological analyses, general considerations, 415
examination of blood, 377
Base of microscope, 50
Basidium, 128
Beer wort, preparation of, 175
Beetroot media, 200
Beggiotoa, morphology of, 133
Benzole bath, 256
Berkefeld filter, 42
Beyrinck's solution I, 197
II, 198
Bile salt agar (MacConkey), 205
broth, double strength, 199
(MacConkey), 180
Biochemical examination of cultures, 276
Biochemistry of bacteria, 276
Biological differentiation of bacteria, 249
Bipolar germination, 140
Bismarck brown, 94
Blastomycetes, morphology of, 129
Blood agar, 171, 214
plates, animal, 251
human, 250
(Washbourn), 214
bacteriological examination of, 377
cells, washing of, 388
collection of, for serological examination, 379
films, preparations of, 376
staining of, 97
histological examination of, 373
pipettes, 11
serological examination of, 378
stains, 97
Blood-serum (Councilman and Mallory), 208
inspissated, 168
(Loeffler), 208
(Lorrain Smith), 208
Blowpipe table, 9
Body tube of microscope, 50
Bohemian flask, 4
Boiling water, 33
Bone marrow, films, preparation of, 400
Bordet-Gengou reaction, 393
Boric acid in milk, test for, 442
Botkin's anaerobic method, 243
Bouillon, preparation of, 163
Brain extract, 149
Bread paste, 193
Brilliant green agar (Conradi), 206
bile salt agar (Fawcus), 206
Brownian movement, 79
Buchner's anaerobic method, 238
Bulloch's anaerobic method, 245
tubes for permanent preparations, 407
Bunge's mordant, 104
Burri's Chinese ink stain, 77
Butter, analysis of, 457
qualitative analysis of, 458
quantitative analysis of, 457
Cadaver, preparation of, for autopsy, 397
Cages for guinea-pigs, 343
for laboratory animals, 341
for mice, 342
for rabbits, 343
for rats, 342
Calculated figure for weight of medium mass, 166, 167
Cambier's candle method of isolating coli-typhoid groups, 438
Camera lucida, 62
Capaldi-Proskauer medium, No I, 186
No II, 187
Capillary pipettes, 10
graduated, 13
Capitate bacilli, 139
Capsule formation, 134
of bacteria, 134
thermo-regulator, 218
Capsules, collodion, inoculation of, 357
preparation of, 357
glass, 6
to clean infected, 20
new, 18
to stain, 99
to sterilise, 31
Carbohydrate media, preparation of, 177
Carbolic acid as a germicide, 27, 481
method of isolating coli-typhoid group, 437
Carbolised agar, 202
bouillon, 202
gelatine, 202
Carbon dioxide in cultures, test for, 289
Card index, 336, 402
Carrot media, 200
Cedarwood oil for immersion lens, 88
Cell wall of bacteria, 134
Celloidin sacs, manufacture of, 358
Cellular incubator, 216
Centrifugal machine for blood and serum work, 327
for milk work, 447
Centrifugalised milk, 449
Centrigade degrees, conversion of, 494
Chemical products of bacteria, 145
China green agar (Werbitski), 207
Chloroform as an antiseptic, 27
Chromatic aberration, 56
Chromogenic bacteria, 131
Chromoparous bacteria, 144
Chromophorous bacteria, 144
Citrated blood agar, 191
Cladothrix, morphology, 193
Classification of bacteria, 131
of fungi, 126
Clavate bacilli, 139
Clearing films with acetic acid, 82
Clostridium, 139
Coarse adjustment, 51
Cobweb micrometer, 66
Cocaine, 441
Experimental infections, study of, during life, 370
inoculation of animals, 332
Extracellular toxins, 144
Eyepiece, Ehrlich, 55
micrometer, 63
Eyepieces, 55
Eye-shade, 57
Fahrenheit degrees, conversion of, 495
Feeding experiments, 369
Fermentation reactions, 279
tubes, 17
Field of objective, 56
Filar micrometer, 66
Filling tubes, etc., with medium, 160
Film preparations, 81
fixing, 81
making, 81
mounting, 85
staining, 83
Filter candle, closed, 47
open, 43
testing efficiency of, 478
to disinfect, 28
to sterilise, 29
flask, 6
papers, to fold, 156
Filters, cotton-wool, 40
porcelain, 42
testing of, 478
Filtration, 40
by aspiration, 42
of media, 156
under pressure, 45
Fine adjustment, 51
spindle head, 52
Fish, analysis of, 460
bouillon, 190
Fish gelatine, 190
gelatine-agar, 190
Fishing colonies, 253
Fission, reproduction by, 136
Fixation, 81
by heat, 81
of tissues, 114
Fixing fluids, for films, 82
Flagella, classification of bacilli by, 136
to stain, 101
Flask Bohemian, 4
Erlenmeyer, 4
filter, 6
Kitasato'a serum, 6
Kolle's culture, 4
Flasks and test tubes, to plug, 24
to clean dirty, 20
new, 18
to sterilise, 31
Fleischwasser, 148
Fluid cultures, description of, 271
media, 146
Foot of microscope, 50
Formaldehyde in milk, Hehner's test for, 442
Formalin method of preserving cultures, 407
tissues, 404
Fractional sterilisation, 33
Fraenkel and Voge's solution, 183
Fraenkel's earth borer, 472
Freezing method for sections, 115
French Mannite Agar (Sabouraud), 193
proof agar (Sabouraud), 193
Fresh preparations of bacteria, 74
FriedlÄnder's capsule stain for sections, 123
Frost's mounting fluid, 406
Frozen sections, rapid method, 116
Fuchsin, 92
agar (Braun), 205
sulphite agar (Endo), 206
Gas analysis, qualitative, 290
quantitative, 290
collecting apparatus, 291
generators, 242
production by bacteria, 289
tubes for media, 161
Gasperini's solution, 193
Gelatin agar, 193
preparation of, 164
surface plates, 231
General anÆsthetics, 345
Gentian violet, 91
German lined paper, 69
Germicides, 27
testing power of, 480
Germination, 140
Geryk air-pump, 43
Glass apparatus in common use, 3
to clean, 18
Glass-cutting knife, 8
Glucose formate agar (Kitasato), 180
bouillon (Kitasato), 180
gelatine (Kitasato), 180
Glycerinated potato, 209
Glycerine agar, 209
blood-serum, 208
bouillon, 209
potato bouillon, 203
broth, 203
Goadby's gelatine, 214
Gonidium, 128
Goniodophore, 128
Graduated capillary pipettes, 13
pipettes, 6
Gram-Claudius' differential stain, 109
Gram's differential stain, 108
Gram-Weigert for sections, 121, 122
Gram-Weigert's differential stain, 109
modified, 110
Grease pencils, 72
Grouping of bacteria for study, 410
Guarded trepine, 360
Guarniari's agar gelatine, 194
Guinea-pig cages, 343
holder, 350
Gulland's solution, 82
Gum solution, preparation of, 116
Guy's culture bottle, 5
Gypsum blocks (Engel and Hansen), 192
HÆmatin, 95
HÆmatocytometer, 248
HÆmatoxilin, 95
HÆmolysin, definition of, 326
preparation of, 327
storage of, 331
HÆmolytic serum, titration of, 328
Hanging-block culture (Hill), 235
Hanging-drop cultures, 233
examination of, 86, 79
preparation of, 78
permanent staining of, 80
slides, 70
Hardening tissues, 114
Haricot agar, 200
bouillon, 200
Hay infusion, 200
Hearson's water bath, 299
Heat effect of, 299
Hehner's test, 442
Heiman's serum agar, 210
Hesse's anaerobic culture method, 237
Histological examination of blood, 373
Holder for guinea-pigs, 350
Hot air, 29
steriliser, 30
to use, 31
incubator, 217
Hot-water funnel, 158
Human blood agar plates, 250
Huyghenian eyepiece, 55
Hydrogen, generating apparatus, 242
in culture, test for, 289
peroxide in milk, test for, 442
Hyphomycetes, morphology of, 126
reproduction of, 126
Ice-box, for water samples, 419
Ice cream, analysis of, 457
Illuminant for microscope, 67
Immune body, 393
Immunisation, methods of, 321
Imperial system, 492
factors for converting, 493
Impression films, 85
Incubators, 216
Index cards, 336, 403
Indol, test for, 286
Infection, definition of, 370
general observations during life, 371
results of, 404
Influence of environment on bacterial growth, 142
Inhalation, fluid inoculum, 365
powdered inoculum, 366
Inhibition coefficient, 310, 311
Inoculation card index, 336
cutaneous, 352
intracranial, 360
intramuscular, 355
intraocular, 362
intraperitoneal, 355
intrapulmonary, 363
intravenous, 363
of collodion capsules, 357
subcutaneous, 353
syringe, 344
Inoculum, character of, 346
preparation of, 346
Inosite-free media—bouillon (Durham), 183
Inseparate toxins, 144
Intermittent sterilisation, 36
Intracellular toxins, 144
Intracerebral inoculation, 362
Intracranial inoculation, 360
Intragastric inoculation, large animals, 367
Marks method, 367
Intramuscular inoculation, 355
Intraocular inoculation, 362
Intraperitoneal inoculation, 355
Intrapulmonary inoculation, 363
Intravenous inoculation, 363
In vacuo anaerobia cultures, 289
Invertin enzymes, tests for, 279
Involution forms, 137
Iodine solution, 108
Iron bouillon, 185
peptone solution (Pakes), 185
Isolation by animal experiments, 258
by differential atmosphere, 257
incubation, 255
media, 255
sterilisation, 256
by dilution, 248
by plate cultures, 250
subcultures, preparation of, 254
Jeffer's counting disc, 424
Jenner's stain, 97
Jores' mounting fluid, 405
Kaiserling fixing solution, 405
Kanthack's serum agar, 211
Killed cultivations, 318
Kipp's hydrogen apparatus, 242
Kitasato's serum flask, 6
Klebs-Loeffler bacillus in milk, 452
Koch's steam steriliser, 34
Kohle's culture flask, 4
Lab enzymes, test for, 279
Laboratory animals, 335
comparative hÆmatocytology of, 374
normal temperature, 372
regulations, 1
Lactose litmus agar (Wurtz), 203
bouillon, 203
gelatine (Wurtz), 203
Lakmus Molke, 203
Lang's solution, 82
Lead bouillon, 185
peptone solution, 186
Leishman's stain, 98
for sections, 125
Lemco broth, 163
Leptothrix, morphology, 133
Lethal dose, minimal, 316
Leviditi's staining method, 124
Light, action of, 308
Liquefiable media, 147
Liquid soap, 346
Lithium carmine, 96
Litmus bouillon, 186
gelatine, 202
milk cultures, description of, 272
preparation of, 172
nutrose agar (Drigalski-Conradi), 205
whey, 195
agar, 196
gelatine, 196
(Petruschky), 195
Local anÆsthetics, 345
reaction to infection, 372
Locomotive movement, 80
Loeffler's capsule stain, (Goadby), 214
proteid free broth (Uschinsky), 183
rosolic acid peptone solutions, 186
serum, bouillon, 210
dextrose water, (Hiss), 188
sugar, (Hiss), 188
water, 170
serum-agar (Heiman), 210
(Kanthack and Stevens), 211
(Libman), 212
(Wertheimer), 211
silicate jelly (Winogradsky), 198
solid, 147
special, 182
stock nutrient, 163
sugar, 177
agar, 185
(dextrose) bouillon, 184
gelatine, 184
sulphindigotate agar, 181
bouillon (Weyl), 181
gelatine (Weyl), 181
tissue (Noguchi), 214
turnip, 200
urine agar, 188
bouillon, 187
gelatine, 187
(Heller), 188
wheat bouillon (Gasperini), 193
whey agar, 195
gelatine, 195
wine must, 192
Winogradsky's solution (for nitric organisms), 198
(for nitrous organisms), 198
wood ash agar, 201
wort agar, 176
gelatine, 176
Media, preparation of yeast water (Pasteur), 191
standardisation of, 154
storage of, in bulk, 159
storing tubes of, 161
sore boxes, 162
titration of, 150
tubing of nutrient, 160
Merismopedia, morphology of, 132
Mesophilic bacteria, 143
pathogenic effects, 315
Metabolic end-products, 145
Metachromatic granules, 136
Metal instruments, to sterilise, 28
Metatrophic bacteria, 131
Methods of cultivation, 221
of identification of bacteria, 259
of inoculation, 352
of isolation, 248
of sterilisation, 26
Methylene-blue, 90
Metric system, 492
factors for converting, 493
Meyer's carmine, 96
Microbes of indication, 426
Micrococci, morphology, 132
Micrococcus, melitensis in milk, 456
Micrometer, filar, 66
net, 63
ocular, 63
stage, 62
Micrometry, methods of, 61
Micron, 61
Microscope, 49
Microscopical examination of bacteria, 86
stained, 88
unstained, 86
observations of cultures, 272
Milk, analysis of, qualitative, 446
quantitative, 444
condensed, analysis of, 444
media, 193
preparation of, 172
rice (Eisenberg), 193
(Soyka), 189
samples, collection of, 443
sedimenting tubes, 449
Minimal lethal dose, 316
Mirror for microscope, 55
Moeller's stain for spores, 107
Moist heat, 32
Molecular movement, 79
Monotrichous bacilli, 136
Motility, examination for, 79
true, 80
Moulds, examination of, 126
for paraffin imbedding, 117, 119
Mounting film preparations, 85
paraffin sections, 119
Mouse cages, 342
holder, 351
scales, 341
Mucor mucedo, 126
MucorinÆ, 126
Mueller's desiccator, 307
Muffle furnace, 28
Muirs's capsule stain, 100
flagella stain, 101
Museum preparations of bacteria, 407
of tissues, 404
sealing of, 406
Mycelium, 126
Mycoprotein, 135
Naegeli's solution, 191
Naehrstoff agar (Hesse and Niedner), 199
Naked flame, 28
Neisser's stain modified, 111
Net micrometer, 63
Neutral litmus solution, preparation of, 179
red, 94
Nitrate bouillon, 185
peptone solution (Pakes), 186
Nitric organisms in soil, 478
Nitrosoindol reaction, 287
Nitrous organisms in soil, 477
Normal averages (t.p.r.), 372
serum, 375
Nosepiece, 57
double, 58
triple, 58
Navy's anaerobic method, 244
jars, 245
Nuclei, to stain, 105
Nucleus of bacteria, 135
Numerical aperture, 56
Nutrient media, 146
Nutrose agar (Eyre), preparation of, 172
Object marker, 61
Objectives, 55
Oblique tube cultures, 223
Ocular micrometer, 63
Oculars, 55
Oese, platinum, 71
OÏdium, 128
Oil of garlic, 27
of mustard, 27
Oleic acid agar (Fleming), 201
Omeliansky's nutrient fluid, 189
Operation tables (Eyre's), 352
(Tatin's), 351
Opsonic index, 393
Opsonic index, determination of, 390
Opsonin, 387
Optical characters of colonies, 267
Optimum reaction of medium, determination of, 305
temperature, determination of, 298
Organisms of suppuration, 409
Orsat-Lunge gas apparatus, 292
Orth's carmine, 96
Oxford stain for Actinomyces, 112
Oysters, analysis of, 463
Pakes' counting disc, 424
filter reservoir, 45
Papier chardin, 158
Pappenheim's stain, 111
Paraboloid condenser, 60
Parachromophorous bacteria, 144
Paraffin method for sections, 117
sections, mounting of, 119
to stain, 121
Paratrophic bacteria, 131
Parietti's bouillon, 202
method of isolating coli-typhoid group, 437
Parsnip medium, 200
Passages of virus, 320
Pasteur-Chamberland filter, 42
Pasteur's pipettes, 10
solution, 191
Pathogenesis, investigation of, 315
Pathogenic bacteria, 131
study of, 408
Pediococci, morphology of, 132
Penicillium, 128
Peptone rosolic acid water, 186
water (Dunham), preparation of, 177
Percentage formula, 496
Perchloride of mercury, 27
PerisporaceÆ, 127
Peritrichous bacilli, 136
Permanent preparations of bacteria, 407
of tissues, 404
Petri's dishes, 6
Phagocytic index, 392
Phenol coefficient, 489
production, test for, 287
Photogenic bacteria, 131, 144
Physiological filter, 156
Picric acid solution, 121
(Spengler's), 112
Picrocarmine, 97
Pigment production, observations on, 288
Pipettes, automatic, 13
blood, 11
capillary, 10
cases for, 7
graduated, 6
capillary, 13
Pasteur's, 10
sedimentation, 16
standard graduated, 7
teat, 10
throttle, 13
to clean infected, 20
new, 18
to ster

26
Stichcultur, 224
Stock dilutions, 497
nutrient media, 163
plate for isolation work, 253
Storage of media in bulk, 159
of tubed media, 161
Store boxes for media, 161
Streak culture, 224
description of, 268
Streaming movement, 80
steam, 35
Streptobacilli, morphology, 133
Streptococci in soil, 477
in water, detection of, 432
morphology of, 132
Streptococcus pyogenes longus in milk, 455
Streptothrix, morphology of, 133
Strichcultur, 223
Structure, internal, of colonies, 265
Study of pathogenic bacteria, 408
Subcutaneous inoculation, 353
Subdural inoculation, 361
Substage condenser, 54
Sugar agar, 185
dextrose bouillon, 184
gelatine, 184
media, preparation of, 177
Sulphindigotate agar, 181
bouillon (Weyl), 181
gelatine (Weyl), 181
Sulphuretted hydrogen in cultures, test for, 290
Sunlight, action of, 309
Superheated steam, 36
Superior lethal coefficient, 310, 313
Suppuration, organisms of, 409
Surface characters of colonies, 264
plates, 230
Surgical motor, electric, 360
Swarm spores, 127
Syringe for subcutaneous inoculation of solid material, 354
hypodermic, 344
Tatin's operating table, 351
Taxonomy, 262
Teat-pipettes, 10
Temperature, action of, 299
optimum, 298
pressure table, 500
range, 298
taking, 340
Test objects for objectives, 57
Testing filters, 478
Test-tubes, 3
to clean infected, 19
new, 18
to plug, 24
to sterilise, 31
Tetracocci, morphology of, 132
Thermal death-point, 143
determination of, 298
of spores, 301, 304
of vegetative forms, 298, 303
Thermophilic bacteria, 143
Thermo-regulators, Hearson's capsule, 218
Reichert's, 218
Thionine blue, 92
Thiothrix, morphology of, 133
Thresh's water collecting bottle, 418
Throttle pipettes, 13
Tinned meat, analysis of, 460
Tissue medium (Noguchi), 214
stains, 95
Tissues for sectioning, fixing, 114
freezing, 116
hardening, 114
imbedding, 118
preparation of, 114
washing, 115
Titration of media, 150
TorulÆ, differentiation from saccharomyces, 130
Total acidity, 280
Toxins, testing of, 318
Trephines, 360
Triple nosepiece, 58
True motility, 80
Tube cultures, preparation of, 222
length, 50
Tubercle bacillus in milk, 453
to stain, 110, 124
Tuberculous guinea-pig, cadaver of, 454
Tubing nutrient media, 160
Turnip media, 200
Unna-Pappenheim's stain for sections, 123
Unsound meat, analysis of, 460
Urine agar, 188
gelatine, 187
(Heller), 188
media bouillon, 187
Uschinsky's solution, 183
Valency of specific sera, 386
Van Ermengem's flagella stain, 104
Vegetative stage of bacteria, 136
Vesuvin, 94
Vibrio cholerÆ in milk, 452
in water, 439
morphology of, 133
Virulence, attenuating, 321
of organisms, 320
raising, 320
Vivisection license, 334
Voges holder, 350
Volatile oils as disinfectants, 27
Warm stage, 58
Washing red blood cells, 388
tissues, 115
Water, analysis of, qualitative, 426
quantitative, 416
steriliser, 33
Weighing animals, 340
Welch's capsule stain, 101
Wertheimer's serum agar, 211
Wheat bouillon (Gasperini), 193
Whey agar, 195
gelatine, 195
Wine must, 192
Winogradsky's solution I, 198
II, 198
Wire crates for test-tubes, 31
Wood ash agar, 201
Working up plates, 252
Wort agar, 176
gelatine, 176
Wright's anaerobic method, 239
Yeast water (Pasteur), 191
Ziehl-Neelsen's stain, 110
Zoogloea, 134
Zymogenic bacteria, 131