CHAPTER II BACTERIA AND PROTOZOA

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BACTERIA

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n the border line between the plant and the animal worlds are many forms which possess some of the characteristics of both. Indeed when an attempt is made to separate all known organisms into two groups one is immediately confronted with difficulties. In looking over the text-books of Botany we will find that certain low forms are discussed there as belonging with the plants, and on turning to the manuals of ZoÖlogy we will find that the same organisms are placed among the lowest forms of animals. The question is of course of little actual importance from certain points of view. It serves, however, to show the close relation of all forms of life, and from a medical standpoint it may be of very great importance owing to the difference in the life-habits, methods of reproduction and methods of transmission of many of the forms that cause disease. We have already seen that none of the diseases that are caused by animal parasites is contagious, while many of those caused by bacteria are both contagious and infectious.

Just over on the plant side of this indefinite border line are the minute organisms known as bacteria. Their numbers are infinite and they are found everywhere. The majority of them are beneficial to mankind in one way or another, but some of them cause certain of the diseases that we will have to discuss later so attention may be called here to a few of the important facts in regard to their organization and life-history in order that we may better understand how they may be so easily transferred from one host to another.

Although these bacilli are so extremely minute (Fig. 7), some of them so small that they cannot be seen with the most powerful microscopes, they differ in size, shape, methods of division and spore-formation. Each species makes a characteristic growth on gelatin, agar or other media upon which it may be cultivated. In this way as well as by the inoculation of animals the presence of the ultramicroscopic kinds may be demonstrated.

The method of reproduction is very simple. They increase to a certain point in size, then divide. This growth and division takes place very rapidly. Twenty to thirty minutes is sufficient time in some cases for a just-divided cell to attain full size and divide again. Thus in a few days time the number of bacteria resulting from a single individual would be inconceivable if they should all develop.

Fortunately for us, however, they do not all multiply so rapidly as this and besides there are natural checks, not the least of which are the substances given off by the bacteria themselves in their growth and development. Such excretions often serve to inhibit further multiplication. Sometimes, though not often, they form spores which not only provide for a more rapid multiplication, but enable the organism to live under conditions that would otherwise prove fatal to it.

Bacteria may be conveniently grouped under two heads: those that live upon dead organic matter, known as the saprophytic forms, and those that are found in living plants or animals, the true parasites. Such a grouping is not always entirely satisfactory, for many of the kinds that live saprophytically under normal conditions may become parasitic if opportunity offers, and also many of those that are usually regarded as parasitic may be grown in cultures of agar or other media, under which conditions they may be regarded as living saprophytically.

It is this power of easily adapting themselves to different conditions that makes many of the kinds dangerous. The bacillus which causes tetanus or lockjaw will illustrate this. It is a rather common bacillus in soil in many localities. As long as it remains there it is of no special importance, but if it is introduced into the body through a scratch or any other wound it becomes a very serious matter.

We may say, then, that the effect the bacillus has on the host depends largely on the host. Not only does it depend on what the host is, but the particular condition of the host at the time of infection is of importance. Children are subject to many diseases that adults seldom have. Hunger, thirst, fatigue, exposure and other factors may make a person susceptible to the actions of certain bacteria that would be harmless under other conditions.

The minute size and great numbers of the bacteria make their dissemination a comparatively simple matter. They may be carried in the air as minute particles of dust; they may be carried in water or milk; they may be carried on the clothing or on the person from one host to another, or they may be disseminated in scores of other ways. In other chapters, particularly the one dealing with the house-fly and typhoid, we shall see how it is that insects are often important factors in spreading some of the most dreaded of the bacterial diseases.

THE PROTOZOA

The Protozoa, or one-celled animals, belonged to an unknown world before the invention of the microscope. The first of these instruments enabled the early observers to see some of the larger and more conspicuous members of the group and each improvement of the microscope has enabled us to see more and more of them and to study in detail not only the structure but to follow the life-history of many of them.

The Amoeba. With some, as the common amoeba (Fig. 8), a minute little form that is to be found in the slime at the bottom of almost any body of water, the life-history is extremely simple. The organism itself consists of a minute particle of protoplasm, a single cell with no definite shape or body-wall and no specialized organs or apparatus for carrying on the life-functions. It lives in the slime or ooze in fresh or salt water, takes its food by simply flowing over the particle that is to be ingested, grows to a certain limit of size, then divides into two more or less equal parts, each part becoming a new animal that goes on with its development as did the parent form. This process of growth and division may go on for many generations, but cannot continue indefinitely unless there is a conjugation of two separate individuals. This process of conjugation is just the opposite to that of division. Two amoeba flow together and become one. It seems to rejuvenate the organism so that it is able to go on with its division and thus fulfil its life-mission which is the same for these lowly animals as with the higher, that of perpetuating the species.

Classes of Protozoa. The group or Phylum Protozoa is divided into four smaller groups or classes. The amoeba belongs to the lowest of these, the Rhizopoda. Rhizopoda means "root-footed," and the name is applied to these animals because most of them move about by means of root-like processes known as pseudopodia or "false feet." This is by far the largest class and contains thousands of forms, mostly living in salt water but there are many fresh-water species. They are non-parasitic, but some of them by their presence in the body may cause such diseases as dysentery, etc.

Fig. 7 Fig. 7—Typhoid Fever bacilli. (After Muir and Ritchie.)
Fig. 8 Fig. 8Amoeba, showing the forms assumed by a single individual in four successive changes. (From Kellogg's Elementary ZoÖl.)
Fig. 9 Fig. 9Euglina virdis. (After Saville Kent.)
Fig. 10 Fig. 10Spirocheta duttoni, × 4500. (After Breinl and Carter.)

The next class which may be known as the whip-bearers (Mastigophora) includes those Protozoa that move by fine undulating processes called flagella. One of the common representatives of this class is the little green Euglena (Fig. 9), whose presence in standing ponds and puddles often imparts a greenish color to the water. Then in the salt water near the surface there are often myriads of minute Noctiluca whose wonderfully phosphorescent little bodies glow like coals of fire when the water is disturbed at night. Although this class contains fewer forms than the preceding some of these have within recent years been found to be of great importance because they live as parasites on man and other animals. The trypanosome whose presence in the blood and tissues of the patient causes that dreadful disease which ends in sleeping sickness belongs here as well as do several other similar kinds that produce serious troubles for various mammals and birds. The SpirochÆta, about which there has been so much recent discussion, also belong here. These are simple spiral-like forms (Fig. 10), that are sometimes classed with the simple plants, bacteria, but Nuttall and others have shown very definitely that they should be classed with the simplest animals, the Protozoans. These are the cause of relapsing fevers in man and of several diseases of domestic animals. It is believed by certain eminent zoÖlogists that when the germ that causes yellow fever is discovered it will be found to belong to this group.

The members of the class Infusoria, so called because they were early found to be abundant in various infusions, are characterized by numerous fine cilia or hair-like organs by means of which the organism moves about and procures its food. The well-known "slipper animalcule" (Paramoecium) (Fig. 11), and the "bell-animalcule" (Vorticella) (Fig. 12) are two common representatives. The Paramoecia were the animals mostly used by Jennings in his wonderfully interesting experiments on the behavior of these lowly forms of life. He showed that they always reacted in a certain definite way in response to particular stimuli, and he was led to believe that he could see "what must be considered the beginnings of intelligence and of many other qualities found in the higher animals." A species of Vorticella was probably the first Protozoan that was ever observed. An old Dutch microscopist, Anton von Leeuwenhoek, in 1675, while studying with lenses of his own manufacture, discovered and described forms which undoubtedly belong to this genus. Few if any of the Infusoria are pathogenic, although some are said to be associated with certain intestinal diseases both in man and the lower animals (Fig. 13).

Fig. 11
Fig. 11
Fig. 12
Fig. 12

Fig. 11Paramoecium. (From Kellogg's Elementary ZoÖl.)

Fig. 12Vorticella, one individual with the stalk coiled, the other with the stalk extended. (From Kellogg's Elementary ZoÖl.)

Fig. 13 Fig. 13—Pathogenic Protozoa; a group of intestinal parasites. A, B, Megastoma entericum, C, Balantidum entozoon. (After Calkins.)

The last class, the Sporozoa, or the spore-forming animals, while small in the number of known species, only about three hundred kinds being known, is extremely important. A number of diseases in man and other animals are due to the presence of these Sporozoans, for they are all parasitic. Few if any animals are exempt from their attacks. They even attack other minute Protozoa. One hundred and fifty-seven species have been recorded as attacking insects, one hundred species attack birds, fifty-two reptiles, eighty crustaceans, twenty-two fish, and so through the list. Ten have been recorded as attacking man. In some instances the parasite is always present in the host and some hosts may harbor several different species of Sporozoa.

Very little work had been done on this group of parasites prior to 1900. Since that time most of the species that we now know have been discovered, and within the last few years the life-histories of many of these have been worked out quite completely. No other group of animals is being studied more to-day by both the physicians and biologists.

The Sporozoa vary greatly in appearance, organization and life-history. They are so very plastic that they can adapt themselves readily to their various hosts, hence we have a great variety of forms. But they all agree in certain characters; all take their food and oxygen and carry on excretory processes by osmosis, i.e., through the body-wall; all are capable of some kind of locomotion, some have one or more flagella, others move by a pseudopod movement. Some are capable of moving from cell to cell in the body as do the white blood-corpuscles. They all agree in the production of spores—hence the name.

At certain stages in their development the nucleus within the body of the organism divides again and again until there are a great many daughter nuclei, each accompanied by a small mass of protoplasm, often inclosed in a little sac or cyst of its own. This is the process of spore-formation and we see that from a single individual we may have by division, not two animals as in the amoeba, but a score or more of them. The little cysts or capsules that inclose them enable them to resist without injury many vicissitudes that would otherwise destroy them. They may dry up or freeze or lie for a long time in the ground or water until the time comes when they are introduced into another host.

The class Sporozoa is divided into five small groups or orders. Nearly all of these contain forms that are of more or less importance, but the ones that live in the blood-cells (HÆmosporidiida) are of the most interest to us because the parasites that cause the malarial fevers and various other diseases belong here. These are dependent on two hosts for their existence, the sexual generation usually occuring in an insect or other invertebrate and the asexual generation in some vertebrate.


                                                                                                                                                                                                                                                                                                           

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