Shiloh National Cemetery was established in 1866 and embraces an area of 10.2 acres. In the cemetery are interred 3,695 bodies, two-thirds of whom are unidentified. Besides the Union soldiers killed in the Battle of Shiloh, the cemetery holds many of the dead from nearby battlefields. In addition, a number of those who served in the Spanish-American War, both World Wars, and one from the Revolutionary War are buried here. Only two Confederates are buried in the cemetery. Both died while being held as prisoners of war.

The Wisconsin Color Guard Memorial is located at the east end of the cemetery on the bluff overlooking the Tennessee River. Another interesting feature of the cemetery is the pyramid of 32-pounder cannon erected by the United States Government to mark the site of the tree used by General Grant as headquarters on the night of April 6.