CHAP. III. A DESCRIPTION OF THE MOST APPROVED MICROSCOPES, AND THE METHOD OF USING THEM.

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CHAP. III. A DESCRIPTION OF THE MOST APPROVED MICROSCOPES, AND THE METHOD OF USING THEM.

In the preceding chapter I have endeavoured to give a comprehensive view of the theory of the microscope, and the principles on which the wonderful effects of this instrument depend. I shall now proceed to describe the various instruments themselves, their apparatus, and the most easy and ready mode of applying them to use; selecting for description those that, from some peculiar advantage in their construction, or from the reputation of the authors who have recommended and used them, are in most general use. What is said of these will, I hope, be sufficient to enable the reader to manage any other kind that may fall in his way.

DESCRIPTION OF ADAMS’S IMPROVED AND UNIVERSAL LUCERNAL MICROSCOPE. Fig. 1. Plate III.

This microscope was originally thought of, and in part executed by my father; I have, however, so improved and altered it, both in construction and form, as to render it altogether a different instrument. The approbation it has received from the most experienced microscopic observers, as well as the great demand I have had for them, has fully repaid my pains and expenses, in bringing it to its present state of perfection.

As the far greater part of the objects which surround us are opake, and very few sufficiently transparent to be examined by the common microscopes, an instrument that could be readily applied to the examination of opake objects, has always been a desideratum. Even in the examination of transparent objects, many of the fine and more curious portions are lost, and drowned as it were in the light which must be transmitted through them; while different parts of the same object appear only as dark lines or spots, because they are so opake, as not to permit any light to pass through them. These difficulties, as well as many more, are obviated in the lucernal microscope; by which opake objects of various sizes may be seen with ease and distinctness; the beautiful colours with which most of them are adorned, are rendered more brilliant, without in the least changing their natural teints. The concave and convex parts of an object retain also their proper form.

The facility with which all opake objects are applied to this instrument is another considerable advantage, and almost peculiar to itself; as the texture and configuration of the more tender parts are often hurt by previous preparation, every object may be examined by this instrument, first as opake, and afterwards, if the texture will admit of it, as transparent.

The lucernal microscope does not in the least fatigue the eye; the object appears like nature itself, giving ease to the sight, and pleasure to the mind: there is also in the use of this instrument, no occasion to shut that eye which is not directed to the object.

A further advantage peculiar to this microscope is, that by it the outlines of every object may be taken, even by those who are not accustomed to draw; while those who can draw well, will receive great assistance, and execute their work with more accuracy, and in less time than they would otherwise have been able to have performed it in. Most of the designs for this work were taken with the lucernal microscope; and I hope the accuracy with which they are executed, will be deemed a sufficient testimony in favour of the instrument. In this point of view it will, I think, be found of great use to the anatomist, the botanist, the entomologist, &c. as it will enable them not only to investigate the object of their researches, but to convey to others accurate delineations of the subject they wish to describe.

By the addition of a tin lanthorn, transparent objects may be shewn on a screen, as by the solar microscope.

Transparent objects may be examined with this instrument in three or four different modes; from a blaze of light almost too great for the eye to bear, to that which is perfectly easy to it.

When this instrument is fitted up in the best way, it is generally accompanied with a small double and single microscope.

Fig. 1. Plate III. represents the IMPROVED LUCERNAL MICROSCOPE, mounted to view opake objects; A B C D E is a large mahogany pyramidical box, about fourteen inches long, and six inches square at its larger end, which forms the body of the microscope; it is supported firmly on the brass pillar F G, by means of the socket H, and the curved piece I K.

L M N is a guide for the eye, in order to direct it in the axis of the lenses; it consists of two brass tubes, one sliding within the other, and a vertical flat piece, at the top of which is the hole for the eye. The outer tube is seen at M N, the vertical piece is represented at L M. The inner tube may be pulled out, or pushed in, to adjust it to the focus of the glasses. The vertical piece may be raised or depressed, that the hole, through which the object is to be viewed, may coincide with the center of the field of view; it is fixed by a milled screw at M, which could not be shewn in this figure.

At N is a dove-tailed piece of brass, made to receive the dove-tail at the end of the tubes M N, by which it is affixed to the wooden box A B C D E. The tubes M N may be removed from this box occasionally, for the convenience of packing it up in a less compass.

O P a small tube on which the magnifiers are screwed.

O one of the magnifiers; it is screwed into the end of a tube, which slides within the tube P; the tube P may be unscrewed occasionally from the wooden body.

Q R S T V X a long square bar, which passes through the sockets Y Z, and carries the stage or frame that holds the objects; this bar may be moved backward or forward, in order to adjust it to the focus, by means of the pinion which is at a.

b e is a handle furnished with an universal joint, for more conveniently turning the pinion. When the handle is removed, the nut, Fig. 2, may be used in its stead.

d e is a brass bar, to support the curved piece K I, and keep the body A B firm and steady.

f g h i is the stage for opake objects; it fits upon the bar Q R S T by means of the socket h i, and is brought nearer to, or removed farther from the magnifying lens, by turning the pinion a; the objects are placed in the front side of the stage, which cannot be seen in this figure, between four small brass plates; the edges of two of these are seen at k l. The two upper pieces of brass are moveable; they are fixed to a plate, which is acted on by a spiral spring that presses them down, and confines the slider with the objects; this plate, and the two upper pieces of brass, are lifted up by the small nut m.

At the lower part of the stage, there is a glass semiglobe n, which is designed to receive the light from the lamp, Fig. 3, and to collect and convey it to the concave mirror o, from whence it is to be reflected on the object.

The upper part, f g r S, of the opake stage takes out, that the stage for transparent objects may be inserted in its place.

Fig. 4. represents the stage for transparent objects; the two legs 5 and 6, fit into the under part r S of the stage for opake objects; 7 is the part which confines or holds the sliders, and through which they are to be moved; 9 and 10 a brass tube, which contains the lenses for condensing the light, and throwing it upon the object; there is a second tube within that, marked 9 and 10, which may be placed at different distances from the object by the pin 11.

When this stage is used as a single microscope, without any reference to the lucernal, the magnifiers or object lenses are to be screwed into the hole 12, and to be adjusted to a proper focus by the nut 13.

N. B. At the end A B of the wooden body there is a slider, which is represented as partly drawn out at A; when quite taken out, three grooves will be perceived, one of which contains a board that forms the end of the box, the next contains a frame with a greyed glass; the third, or that farthest from the end A B, two large convex lenses.

OF THE LAMP.

Fig. 3, represents one of Argand’s lamps, which is the most suitable for microscopic purposes, on account of the clearness, the intensity, and the steadiness of the light. The following method of managing it, with other observations, is copied from an account given by Mr. Parker, with those he sells.

The principle on which the lamp acts, consists in disposing the wick in thin parts, so that the air may come into contact with all the burning fuel, by which means, together with an increase of the current of air occasioned by rarefaction in the glass tube, the whole of the fuel is converted into flame.

The wicks are circular, and, the more readily to regulate the quantity of light, are fixed on a brass collar with a wire handle, by means of which they are raised or depressed at pleasure.

To fix the wick on, a wood mandril is contrived, which is tapered at one end, and has a groove turned at the other.

The wick has a selvage at one end, which is to be put foremost on the mandril, and moved up to the groove; then putting the groove into the collar of the wick-holder, the wick is easily pushed forward upon it.

The wick-holder and wick being put quite down in their place, the spare part of the wick should, while dry, be set alight, and suffered to burn to the edge of the tubes; this will leave it more even than by cutting, and, being black by burning, will be much easier lighted: for this reason, the black should never be intirely cut off.

The lamp should be filled an hour or two before it is wanted, that the cotton may imbibe the oil, and draw the better.

The lamps which have a reservoir and valve, need no other direction for filling, than to do it with a proper trimming pot, carefully observing when they are full; then pulling up the valve by the point, the reservoir being turned by the other hand, may be replaced without spilling a drop.

Those lamps which fill in the front like a bird-fountain, must be reclined on the back to fill, and this should be done gently, that the oil in the burner may return into the body when so placed and filled; if, by being too full, any oil appear above the guard, only move the lamp a little, and the oil will disappear; the lamp may then be placed erect, and the oil will flow to its proper level.

The oil must be of the spermaceti kind, commonly called chamber oil, which may generally be distinguished by its paleness, transparency, and inoffensive scent; all those oils which are of a red and brown colour, and of an offensive smell, should be carefully avoided, as their glutinous parts clog the lamp, and the impurities in such oil not being inflammable, will accumulate and remain in the form of a crust on the wick. Seal oil is nearly as pale and sweet as chamber oil, but being of a heavy sluggish quality, is not proper for lamps with fine wicks.

Whenever bad oil has been used, on changing it, the wick must also be changed, because, after having imbibed the coarse particles in its capillary tubes, it will not draw up the fine oil.

To obtain the greatest degree of light, the wick should be trimmed exactly even, the flame will then be completely equal.

There will be a great advantage in keeping the lamp clean, especially the burner and air tubes; the neglect of cleanliness in lamps is too common: a candlestick is generally cleaned every time it is used, so should a lamp; and if a candlestick is not to be objected to, because it does not give light after the candle is exhausted, so a lamp should not be thought ill of, if it does not give light when it wants oil or cotton; but this last has often happened, because the deficiency is less visible.

The glass tubes are best cleaned with a piece of wash leather.

If a fountain lamp be left partly filled with oil, it may be liable to overflow; this happens by the contraction of the air when cold, and its expansion by the warmth of a room, the rays of the sun, or the heat of the lamp when re-lighted: this accident may be effectually prevented by keeping the reservoir filled, the oil not being subject to expansion like air. On this account, those with a common reservoir are best adapted for microscopic purposes.

TO EXAMINE OPAKE OBJECTS WITH THE LUCERNAL MICROSCOPE.

The microscope is represented as mounted, and entirely ready for this purpose, in Fig. 1. Plate III.

To render the use of this instrument easy, it is usually packed with as many of the parts together as possible; it occupies on this account rather more room, but is much less embarrassing to the observer, who has only three parts to put on after it is taken out of its box, namely, the guide for the eye, the stage, and the tube with its magnifier.

But to be more particular, take out the wooden slide A, then lift out the cover and the grey glass from their respective grooves under the slide A.

Put the end N of the guide for the eye L M N into its place, so that it may stand in the position which is represented in this figure.

Place the socket, which is at the bottom of the opake stage, on the bar Q X T, so that the concave mirror o may be next the end D E of the wooden body.

Screw the tubes P O into the end D E. The magnifier you intend to use is to be screwed on the end o of these tubes.

The handle G b, or milled nut, Fig. 2, must be placed on the square end of the pinion a.

Place the lamp lighted before the glass lump n, and the object you intend to examine between the spring plates of the stage, and the instrument is ready for use.

In all microscopes, there are two circumstances which must be particularly attended to; the modification of the light, or the proper quantity to illuminate the object; secondly, the adjustment of the instrument to the focus of the glasses and the eye of the observer. In the use of the lucernal microscope there is a third circumstance, which is the regulation of the guide of the eye, each of which I shall consider by itself.

1. To throw the light upon the object. The flame of the lamp is to be placed rather below the center of the glass semiglobe n, and as near it as possible; the concave mirror o must be so inclined and turned, as to receive the light from the semiglobe; and reflect it thence upon the object; the best situation of the concave mirror, and the flame of the lamp, depends on a combination of circumstances, which a little practice will best point out.

2. To regulate the guide for the eye, or to place the center of the eye piece L, so that it may coincide with the focal point of the lenses, and the axis of vision. Lengthen and shorten the tubes M N by drawing out or pushing in the inner tube, and raising or depressing the eye-piece M L, till you find the large lens, which is placed at the end A B of the wooden body, filled by an uniform field of light, without any prismatic colours round the edge; for, till this piece be properly fixed, the circle of light will be very small, and only occupy a part of the lens; the eye must be kept at the center of the eye-piece L, during the whole of the operation; which may be rendered somewhat easier to the observer, on the first use of the instrument, if he hold a piece of white paper parallel to the large lenses, removing it from or bringing it nearer to them, till he finds the place where a lucid circle, which he will perceive on the paper, is brightest and most distinct, then to fix the center of the eye-piece to coincide with that spot; after which a very small adjustment will set it perfectly right.

3. To adjust the lenses to their focal distance. This is effected by turning the pinion a, the eye being at the same time at the eye-piece L. I often place the grey glass before the large lenses, while I am regulating the guide for the eye, and adjusting for the focal distance.

If the observer, in the process of his examination of an object, advance rapidly from a shallow to a deep magnifier, he will save himself some labour by pulling out the internal tube at O.

The upper part f g r s of the stage, is to be raised or lowered occasionally, in order to make the center of the object coincide with the center of the lens at O.

To delineate objects, the grey or rough ground glass must be placed before the large lenses; the picture of the object will be formed on this glass, and the outline may be accurately taken, by going over the picture with a pencil.

The opake part may be used in the day-time without a lamp, provided the large lenses at A B be screened from the light.

TO USE THE LUCERNAL MICROSCOPE IN THE EXAMINATION OF TRANSPARENT OBJECTS.

The microscope is to remain as before: the upper part f g r s of the opake stage must be removed, and the stage for transparent objects, represented at Fig. 4, put in its place; the end, Fig. 9 and 10, to be next the lamp.

Place the rough glass in its groove at the end A B, and the objects in the slider-holder at the front of the stage; then transmit as strong a light as you are able on the object, which you will easily do, by raising or lowering the lamp.

The object will be beautifully depicted on the rough glass: it must be regulated to the focus of the magnifier, by turning the pinion a.

The object may be viewed either with or without the guide for the eye; a single observer will see an object to the greatest advantage by using this guide, which is to be adjusted as we have described, page 73. If two or three wish to examine the object at the same time, the guide for the eye must be laid aside.

Take the large lens out of the groove, and receive the image on the rough glass; in this case the guide for the eye is of no use: if the rough glass be taken away, the image of the object may be represented on a paper screen.[29]

[29] A tin cover is sometimes made to go over the glass chimney of the lamp, Fig. 3, with only a small square aperture in front, sufficient to suffer the rays to pass into the microscope: this, by excluding all extraneous rays, adds in many cases most materially to the effect, particularly by day, and when objects are to be represented on the rough glass or screen only. Edit.

Take out the rough glass, replace the large lenses, and use the guide for the eye; attend to the foregoing directions, and adjust the object to its proper focus. You will then see the object in a blaze of light almost too great for the eye, a circumstance that will be found very useful in the examination of particular objects; the edges of the object in this mode will be somewhat coloured, but as it is only used in this full light for occasional purposes, it has been thought better to leave this small imperfection, than by remedying it, to sacrifice greater advantages; the more so, as this fault is easily corrected, and a new and interesting view of the object is obtained, by turning the instrument out of the direct rays of light, and permitting them to pass through only in an oblique direction, by which the upper surface is in some degree illuminated, and the object is seen partly as opake, partly as transparent. It has been already observed, that the transparent objects might be placed between the slider-holders kl of the stage for opake objects, and then be examined as if opake.

Some transparent objects appear to the greatest advantage when the lens at 9 and 10 is taken away; as, by giving too great a quantity of light, it renders the edges less sharp.

The variety of views which may be taken of every object, by means of the improved lucernal microscope, will be found to be of great use to an accurate observer: it will give him an opportunity of correcting or confirming his discoveries, and investigating those parts in one mode, which are invisible in another.

TO TRANSMIT THE IMAGE OF TRANSPARENT OBJECTS ON A SCREEN, AS BY THE SOLAR MICROSCOPE.

It has been long a microscopical desideratum, to have an instrument by which the image of transparent objects might be shewn on a screen, as by the common solar microscope; and this not only because the sun is so uncertain in this climate, and the use of the solar microscope requires confinement in the finest part of the day, when time seldom hangs heavy on the rational mind, but as it also affords an increase of pleasure, by displaying its wonders to several persons at the same instant, without the least fatigue to the eye.

This purpose is now effectually answered, by affixing the transparent stage, Fig. 4, of the lucernal to a lanthorn containing one of Argand’s lamps. The lamp is placed within the lanthorn, and the end 9, 10 of the transparent stage is screwed into a female screw, which is rivetted in the sliding part of the front of the lanthorn; the magnifying lenses are to be screwed into the hole represented at 12; they are adjusted by turning the milled nut. The quantity of light is to be regulated, by raising and lowering the sliding plate, or the lamp. N. B. This part, with its lanthorn and lamp, may be had separate from the lucernal microscope.[30]

[30] This effect by the lanthorn and lamp is subject to much limitation in the field of view, or circle of light thrown upon the screen. A circle of not more than from 12 to about 15 inches can ever be obtained with any tolerable strength of light, to shew the most transparent sort of objects that can be found, such as the scale of a sole fish, a fly’s wing, &c. The great difference between the light of the sun and a lamp is a natural obstacle to great performances in this way, and renders them far short of the effects of the solar microscope. The exhibition, however, is considerable, and much deserving of the notice of any observer disposed to this sort of apparatus. Probably, subsequent experiments may yet produce more light on this instrument. The best sort of apparatus for this purpose hitherto made, I shall describe in a following section. Edit.

APPARATUS WHICH USUALLY ACCOMPANIES THE IMPROVED LUCERNAL MICROSCOPE.

The stage, Fig. 1, f g h i, for opake objects, with its glass semiglobe, and concave mirror, which is moveable upon the bar Q R S T, and set readily to any distance by the screw at a. The glasses o and n are also moveable upon the bar for regulating and adjusting the light upon the object.

The stage, Fig. 4, for transparent objects, which fits on the upper part P S of the foregoing stage. When this is to be applied occasionally to a lanthorn for shewing transparent objects on a screen, &c. it is made of a much larger diameter, to admit of the illuminating lenses at 9, 10, and 11, of greater power of condensing the rays from the lamp.

The sliding tube O P, to which the magnifiers are to be affixed; one end of this is to be screwed on the end B of the wooden body; the magnifier in use is to be screwed to the other end on the inner tube. This tube slides inwards or outwards; it is first used to set the magnifier at nearly the right distance from the object, before the exact adjustment for the focus is made, by turning the pinion at a with Hook’s joint and handle b e.

Eight magnifying lenses in brass cells, Fig. 5. Plate III. these are so constructed that any two of them may be combined together, and thus produce a very great variety of magnifying powers. The cells unscrew to admit of the glasses being cleaned.

A fish-pan, such as is represented at Fig. 6, whereon a small fish may be fastened in order to view the circulation of its blood; its tail is to be spread across the oblong hole at the smallest end, and tied fast by means of the attached ribbon. The knob on its back is to be put through a slit hole on the brass piece, No. 5, of Fig. 4. The tail of the fish is to be brought then immediately before the magnifier.

A steel wire, Fig. 7, with a pair of nippers at one end, and a steel point at the other; the wire slides backwards or forwards, in a spring tube which is affixed to a joint at the bottom, on which is a pin to fit the hole in the leg, No. 6, Fig. 4. This is used to confine small objects.

A slider of brass, Fig. 8, containing a flat glass slider and a brass slider, into which are fitted some small concave glasses. It is for confining small living objects, and when used is placed between the two plates, No. 7, Fig. 4.

A pair of forceps, Fig. 9, by which any occasional small object may be conveniently taken up.

Six large ivory sliders, with transparent objects placed between two plates of talc, and confined by brass rings, and six small ditto with ditto. Fig. 10. The larger ones usually contain a set of Custance’s fine vegetable cuttings.

Fourteen wood sliders, containing on each four opake objects, and two spare sliders for occasional objects; all fitted to the cheeks kl of the stage. Fig. 11.

Some capillary tubes, Fig. 12, to receive small fish, and for viewing small animalcula. They are to be placed between the two plates of the stage No. 7, Fig. 4.

A small ivory double box, containing spare plates of talc and brass rings, for replacing any in the small ivory sliders, when necessary.

A single lens mounted in a tortoiseshell case, to examine minute objects previous to their being applied to the sliders.

Opake objects are easily put on the spare sliders by a wetted wafer; and, for good security, gum water may be added.

For the prices of the lucernal, as well as all the other sorts of microscopes, see the list annexed to these Essays.

A DESCRIPTION OF THE SEVERAL IMPROVEMENTS MADE UPON MR. ADAMS’S LUCERNAL MICROSCOPE. BY THE EDITOR.

The lucernal microscope being unquestionably the only instrument for exhibiting all sorts of opake objects under a brilliant and magnified appearance, was, as formerly constructed by the late Mr. G. Adams, attended with some inconveniences and imperfections. Upon a proper inquiry into various improvements, and from some observations made by myself, I can recommend, as a complete instrument, one with the following emendations, being, in my opinion, the best of any hitherto made known.

The lucernal microscope, when placed up for use, as represented in Fig. 1. Plate III. is of some considerable length. When the eye at L is viewing the image of the object upon the glasses, the objects themselves in the sliders placed at kl at the stage, are without the reach of the hand; so that the indispensible change of the parts of an object, or of one object to another, can only be obtained by the observer’s moving himself from the object to the eye-piece, and vice versa. This adjustment, therefore, proves uncertain and troublesome. The application of rack-work motion to the stage has been contrived and applied to the lucernal microscope by Mr. W. Jones, of Holborn, accompanied with Hooke’s joint and handle, and a lever rod; so that, without altering his position, the observer may change both the horizontal and vertical position of the sliders, and thereby readily investigate all the variety of the objects, and their parts, and with the same exactness as by other microscopes.

For persons who may not wish to be at the expense of the lucernal, as formerly mounted by Mr. Adams, Mr. Jones has altered the manner of its support; which, as well as the other particulars, and the method of using it, may be understood from the following description.

Plate IX. Fig. 3, is a representation of the instrument as placed up for use. AA, the top of a mahogany chest, about two feet long, thirteen inches and an half high, and eight inches broad, which serves both as a case to contain the instrument, and to support and preserve it steady when in use. A groove is cut in the top of the box, and another in the inside at the bottom, in both of which the base of the instrument is made to slide. When the instrument is placed inside, a long slip of mahogany slides in at the top, to secure the groove, and make the top perfect. Thus the instrument may be most readily slid out of its case, and then into the groove at top for use, and in much less time than by the brass frame and jointed stand adopted by Mr. Adams. Fig. 3 B, is the stage for the objects, with the condensing lens a, and concave mirror b, the same as in Mr. Adams’s. C, the brass slider case for opake objects, with a rack cut into its lower edge, and which is turned by a pinion. To this pinion is applied an handle, D, with Hooke’s universal joint; this contrivance gives a certain horizontal motion to the objects while viewing. The stage at C is also made to slide vertically, and a lever-rod or handle, E, to apply through the top, to bring the objects to a just height. Hence, by applying the left hand to the handle, E, and the right to the rod D, the adjustment or the changing of the objects, while under exhibition on the large lenses at F, is produced in the most convenient and accurate manner, and the observer has no occasion, for one slider, to shift from his seat or position.

Rack-work might be applied to the vertical motion, but it is not essentially necessary; for when once the center of the slider is observed, there requires very little change from that position for the complete exhibition of the objects. The whole of the stage, with the lense and mirror, is fixed to a brass dove-tailed slider at G, which slides in another brass piece fixed to the wooden slider or base of the instrument. A long brass rod, H, with an adjusting screw at its end, passes through the two brass pillars, K, K, to the stage at f, upon which it acts; and according as it is turned to the right or left hand while examining the objects, moves the objects nearer to or farther from the magnifiers screwed on at L, and produces the just distance for rendering the appearance of the objects the most distinct and brilliant upon the glasses at F.

The management of the light from the lamp, through the lens, a, and from the concave mirror, b, to the objects, is exactly the same as before directed by Mr. Adams. For the exhibiting of transparent objects, the stage, C, is to be slid away, and the body, Fig. 4, applied in its place, in that position, with the large lens outwards next the lamp. The slider with the objects passes through at a, and the focus for the different magnifiers is adjusted by turning the long rod, D, to the right or left, as with the opake objects. In this case the lamp is to be raised to the center of the body of the microscope, or even with the magnifiers at L. The image of the objects may also, as in Mr. Adams’s, be best received on the rough glass placed at F, for the simple reflected light through the body will sometimes be so strong, as to irritate the eye; the operator must, therefore, both modify that from the lamp, and place the roughed glass to his own ease and pleasure. The guide for the eye, N, in this instance is not necessary. Care being taken that the roughed glass at F be kept in as dark a situation as possible, there will be a certainty of a clear and well-defined view of the object.

A tin chimney placed over the glass of the lamp about ten inches long, with a suitable aperture to admit the light to pass through it to the glasses, is of material service; it excludes all superfluous light from the eye of the observer, keeps the room sufficiently darkened, and enables the observer to view his object with the proper brilliancy. As a pleasing relief to the eye, the interposition of a small piece of blue or green glass at the sight hole, N, Mr. Jones has sometimes found necessary, but it gives rather a false teint to the colour of the objects.

In the year 1789 the same artist applied a brass screw pillar and arm to the top of the box at O, on which is occasionally slid the condensing lens, a. The lamp being then applied at the side of the box at O, instead of the end, and the lens, a, moved to such a distance as to give the strongest possible light upon the opake objects at C; they were found to be more strongly illuminated by this simple refracted light than by the refracted and reflected light before used. Light is always somewhat diminished by reflection, although condensed; therefore, as it is sometimes best to view the objects from oblique reflected light, and sometimes from direct refracted, he constructs the apparatus so as to give the operator the means of easily using either. The dotted lines, O P, shew the manner that the glass semiglobe, a, is occasionally applied to refract or converge directly the light from the lamp to the objects on the stage.

It is scarcely necessary to observe to the reader, the propriety of all the glasses of the apparatus being perfectly clean before the observations; for if, after being laid aside some time, or by dust, &c. they should appear soiled, it will be necessary to wipe them previously with a piece of soft shammy leather usually sent in the box for that purpose, or a clean soft cloth. The two large lenses at F, Fig. 3, may be readily separated by turning aside the two brass screws that act upon a brass ring.

From the various ingenious admirers of this sort of instrument, many improvements and alterations have been suggested; among several that have been communicated, those by the two following gentlemen appear to me the most deserving of notice, and which I shall leave to the reader’s judgment and experience.

The Rev. John Prince, LL. D. now of Salem, Massachuset’s States, North America, a valuable correspondent and friend of our late author, transmitted to him an alteration in the construction; and of which I here insert the brief account, in nearly the words given by Mr. Adams.

Dr. Prince applies a strong joint similar to that of a telescope at about the middle of the center part of the pyramidical box, and a sort of adjusting screw at the large end. The joint is nearly in the center of gravity, so that a very small motion is sufficient to bring any object less than an inch in diameter into the field of view. This motion is effected by two screws at right angles to each other; one screw raising or levelling the body, the other moving it sidewise, the screw at the same time forming a double joint to accommodate the parts to the movement.[31]

[31] A figure of this, with an explanation, as recommended by Mr. John Hill, Wells, in Norfolk, may be seen in the Gentleman’s Magazine, Vol. LXVI. 2d part, page 897. In this particular, as well as in the deviation from the parallel position of the glasses to the surfaces of the objects, I think the construction not so simple and perfect, as that by rack-work and pinion applied by Mr. Jones. Probably, Dr. Prince had not, at the time of his contriving the joint-work to the box, seen or heard of the other method. His subsequent contrivances shew real ingenuity; and to the inquisitive in this instrument, will afford much useful entertainment and advantage.

To secure the image formed upon the rough glass more completely from the light, at times essentially necessary, there is a pyramidical mahogany box, of the same size, to pack, when not used, in the body of the microscope; when in use, the broad end of this screen box is to be slid into the groove, from which the exterior cover at the end has been taken. This method is peculiarly useful in the day-time; as, by screening the large lenses from the light, it may even then be used with satisfaction.

One of the large lenses may occasionally be placed on the outer edge of the screen box, the other lens being taken out; the view on the rough glass is by these means magnified, and appears to greater advantage. But, besides the grey glass used in the former construction, there is a second in this, placed farther within the body, about half way; and, when the large lens is in the screen box, objects appear better in this than in the former way. It has a still greater effect upon those who are unacquainted with the nature of lenses, as it makes them judge the distance and magnitude much greater than they really are, and is therefore more pleasing than the grey glass in front. Only one grey glass can be used at a time; both being removed when opake objects are viewed.

The stage, F, Fig. 5, is considerably different from that at C, Fig. 3; it is judged more convenient and commodious than the other, and serves, with a small alteration, for both transparent and opake objects. A truncated cone can also be here applied for cutting off superfluous rays of light occasionally.

The method of illuminating the objects is also different. The mode now adopted answers better for opake and transparent objects, throws a stronger light, and is more convenient in application. It consists of two lenses, 1 and 2, Fig. 5; the larger one is to be placed at the end of the bar next the lamp. The smaller one to be adjusted so as to give a strong light. A third is also added, to be used occasionally with opake objects; it is to be applied close to the large lens. Experience will shew when it is to be used, or not. By moving the bar, G, on which these lenses are placed round the stage pillar, M, you bring it so much fronting the stage as effectually to enlighten opake objects by means of the lamp. The light thus afforded is received directly, and none lost by reflection. The objects are fixed on circular wheels of wood, see Fig. 7, the brass centers of which, are fitted to the hole, b, of the stage, Fig. 5; and about this center they are to be turned by the hand for the changing of the objects.

As some objects, such as sections of wood, are seen to advantage both as transparent and opake, a frame containing a plane and a concave mirror is added to this instrument, serving two purposes: by bringing the bar to the front of the stage, removing the large lens, and putting the mirror in its place, the object may be viewed either way, without moving from the seat, by turning the instrument a little round. This experience will discover. The light of the sun may be thrown by the plane mirror on the condensing lens, so as to produce a strong full field of light on the grey glass. This has a grand effect when the large lens is at the end of the screen box, and could not be applied in this manner in former constructions. It becomes also an opake solar microscope, by turning the bar round to enlighten opake objects.

By bringing the concave mirror to a focus that will burn objects, a set of very curious and entertaining experiments may be made and exhibited on the grey glass. The object for combustion should be put in the nippers, and a piece of slate tied as a ground on the stage. The ebullition of a piece of alum viewed in this manner is very beautiful; the bubbles, as they rise and pass off rapidly, appear tinged with all the colours of the rainbow.

There are large-sized magnifiers for the purpose of throwing transparent objects on a screen, in imitation of the solar microscope. By removing the large lenses in front and the grey glass, and placing the black tin cylinder represented in the drawing by dotted marks, over the lamp, they may be shewn in that manner to several persons; thus, this instrument in a great degree supersedes the use of a lanthorn. The image may be contracted occasionally by one of the large lenses.

The following improvement consists in the manner of applying the lamp, by Mr. Hill. By attaching it to the instrument, it renders the light more permanent and steady, and reduces considerably the bulk as well as the trouble of this appendage, and is to be preferred when the lamp is not wanted separately for other uses or experiments.

H, a brass support to the arm, G, for sustaining the weight of the lamp; it turns round with the bar on the pillar, M. At about I is a brass cap soldered to the above support, and which slips over the slider carrying the larger lens, 2. At K, is a strong joint connected with the said cap, and by which an horizontal motion of the cap is given, when an oblique light is required. To the end of this the lamp is fixed, and in such a manner as to admit of its being easily slid upwards or downwards in a perpendicular direction, to procure the just height of the flame. L is a square brass rod to be occasionally screwed into the reservoir of the lamp, for supporting the tin cylinder screen, when transparent objects are to be represented on a screen in a darkened room.

The transparent microscope, part of the lucernal, is sometimes adapted to a large japanned tin lanthorn, such as represented at Fig. 6. A brass female screw is soldered to the front of the lanthorn, which has a motion upwards or downwards, fitted to the male screw of the transparent microscope. A tall chimney is placed at the top of the lanthorn to conduct the heated air from an Argand’s lamp withinside. The transparent objects in the sliders are magnified by the lenses screwed on at a, and shewn on the screen A; this screen may be about three feet square, of white paper, the objects on which, if represented in a field larger than twelve or eighteen inches, will not be sufficiently vivid.

Mr. Jones has found that a large square glass, from twelve to sixteen inches in the side, rough ground on one of its surfaces, exhibits the objects the best of any other contrivance; answers tolerably well for opake objects, and gives the artist the means of tracing their figure most correctly on its surface. Such sort of objects he fixes upon slips of glass for that purpose, or applies them to a pair of nippers shewn at b, sent with the microscope. A concave silver speculum screws on at c, before the magnifiers, which reflects upon the objects the light that issues from the lamp through the body of the microscope. The least dimensions of the lanthorn are about ten inches square, and fourteen inches high.

This microscope and lanthorn, when made as a separate apparatus from the lucernal, is called the LANTHORN MICROSCOPE. Its effect is considerably short of what is produced by the solar microscope, and not equal to what is much wished for in this manner of magnifying minute objects; see note, page 77.

Partly from the improvements just described, Mr. Jones is now constructing a lucernal microscope that he conceives will be the most simple and perfect yet made. It could not be completed in time to be described in this work; but its improvement and advantages will be quite evident to any reader who has attended to the description which I have just given.

DESCRIPTION OF CUFF’S DOUBLE-CONSTRUCTED MICROSCOPE, REPRESENTED AT Fig. 1. Plate VII. A.[32]

[32] The compound or double microscope is in more general use than any other sort. Besides its being less expensive than the lucernal or complete solar, it is found commodious and portable in the observer’s apartment, when only a confined degree of microscopical pursuit is intended, and that chiefly for a few hours amusement; it may be used both by day and night. In the most improved of this kind the objects appear magnified in a field of view from about 12 to 15 inches in diameter. It is better adapted to transparent than to opake objects, yet the latter may often be viewed to great advantage by the assistance of the sun’s rays or the light of a candle condensed on them. The intelligent reader, by attending to the accounts of the different microscopes described in this work, will be enabled to select that best adapted to the kind of objects he wishes to explore, and the manner in which he is desirous of having them exhibited. Edit.

This instrument was first described by Mr. Baker, and recommended by him. It was also described by my father in the fourth edition of his Micrographia Illustrata, page xix.

A B C represents the body of this microscope; it contains an eye-glass at A, a large lens at B, and a magnifier which is screwed on at C, one of which is represented at Q.

The body of the microscope is supported by the arm D E, from which it may be removed at pleasure.

The arm D E is fixed on the sliding bar F, and may be raised or depressed to any height within its limits.

The main pillar a b is fixed in the box b e, and by means of the brass foot d is screwed to the mahogany pedestal X Y, in which is a drawer containing all the apparatus.

O, a milled-headed screw, to tighten the bar F when the adjusting screw c g is used.

p q is the stage or plate which carries the objects; it has a hole at the center n.

G, a concave mirror, that may be turned in any direction, to reflect the light of the candle, or the sky, upon the object.

A LIST OF THE APPARATUS TO CUFF’S DOUBLE-CONSTRUCTED MICROSCOPE.[33]

[33] This microscope is made oftentimes with a joint at the bottom of the main pillar at e, to admit placing the instrument into any oblique situation, and connected to the bottom of a mahogany chest; on which account, it is by some of the instrument makers called the Chest Compound Microscope. Edit.

H, a convex lens, to collect the rays of light from the sun or a candle, and condense them on the object, or to magnify a flower or other large object placed upon the stage.

L, a cylindrical tube, open at each side, with a concave silver speculum screwed to the lower end h.

P, the slider-holder; it consists of a cylindrical tube, in which an inner tube is forced upwards by a spiral spring, it is used to receive an ivory slider K, which is to be slid between the plates h and i. The cylinder P fits the hole n in the stage: the hollow part at k is designed to receive a glass tube N.

R is a brass cone, to be put under the bottom of the cylinder P, to intercept occasionally some of the rays of light.

S, a box containing a concave and a flat glass, between which a small living insect may be confined; it is to be placed over the hole n.

T, a flat glass to lay any occasional object upon; there is also a concave one u, for fluids.

O, a long steel wire, with a small pair of pliers at one end, and a point at the other, designed to stick or hold objects; it slips backwards and forwards in the short tube o; the pin p fits into an hole m, in the stage for that purpose.

W, a little round ivory box, to hold a supply of talc and rings for the sliders.

Z, a hair brush, to wipe any dust off the glasses, or to take up by the other end a drop of any liquid.

V, a small ivory cylinder, that fits on the pointed end of the steel wire O; it is designed for opake objects. Light-coloured ones are to be stuck upon the dark side, and vice versa.

Y, a common magnifying glass for any occasional purpose.

M, a fish-pan whereon to fasten a small fish, to view the circulation of the blood: the tail is to be spread across the oblong hole at the small end k, and tied fast by means of a ribband fixed thereto; the knob l is to be put through the slit made in the stage, and the tail may be brought under the magnifier.

X is a wire to clean the glass tubes by.

TO USE THIS MICROSCOPE.

Screw the magnifier you intend to use to the end C of the body, place the slider-holder P in the hole n, and the ivory slider K with the object, between the plates h i of the slider-holder; set the upper edge of the bar D E to coincide with the division which corresponds to the magnifier you have in use, and tighten it by the milled nut O; now reflect a proper quantity of light upon the object, by means of the concave mirror G, and regulate the body exactly to the eye and the focus of the glasses by the adjusting screw c g, at the same time you are viewing the object.

To view opake objects, take away the slider-holder P, and place the object on a flat glass u, under the center of the body, or on one end of the jointed nippers o. Then screw the silver concave speculum to the end of the cylinder L, and slide this cylinder on the lower part of the body, so that the upper edge thereof may coincide with the line which has the same mark with the magnifier that is then used; reflect the light from the concave mirror G to the silver speculum, from which it will be again reflected on the object. The glasses are to be adjusted to their focal distance as before directed.

THE DESCRIPTION AND USE OF JONES’S IMPROVED COMPOUND OR DOUBLE MICROSCOPES, REPRESENTED IN Fig. 1 AND 2. Plate IV. BY THE EDITOR.

The chief imperfections of Cuff’s microscope, as well as of others formerly made, are, their construction rendering them only compound microscopes, the body of the instrument having but a fixed position over the object, and the smallness of the field of view by the old construction of the glasses in the body. To obviate these defects, as well as for the application of material improvements, the late Messrs. Martin and Adams, and the present Messrs. W. and S. Jones, have constructed this kind of microscope in various ways. Two microscopes by the latter artists, which I am now going to describe, appear to me to be the best of any hitherto invented.

Fig. 1 is a representation of the second best sort of compound microscopes. The improvements, though few in number, are essential to the use thereof. The field of view is considerably larger than in the former microscope. The stage and the mirrors are both moveable, so that their respective distances may be easily varied. The magnifiers may be moved about over the object. There is also a condensing glass, for increasing the density of the light, when it is reflected by the mirror from a candle or lamp. It is furnished with two mirrors, one plane and the other concave, and may likewise be used as a complete single microscope.

A B, Fig. 1. represents the body of the microscope, containing a double eye glass, and a body glass; it is here shewn as screwed to the arm C D, from whence it may be occasionally removed, either for the convenience of packing, or when the instrument is to be used as a single microscope.

The eye glasses and the body glasses are contained in a tube which fits into the exterior tube A B; by pulling out a little this tube, when the microscope is in use, the magnifying power of each lens is increased.

The body A B of the microscope is supported by the arm C D; this arm is moveable in a square socket cut in the head that is connected to the main pillar E F, which is screwed firmly to the mahogany pedestal G H; there is a drawer to this pedestal, which holds the apparatus. This arm may be slid backwards and forwards in its socket, carrying the magnifiers and the body of glasses, and also turned horizontally quite round upon the pillar, giving a general motion all over the object on the stage below; which is a material improvement and advantage of this microscope over a similar one described in the former edition of this work, as any unavoidable motion of the living object to be viewed may be followed, by the observer’s hand moving the arm C D as the object changes its place.

N I S is the plate or stage which carries the slider-holder K, this stage is moved up or down the pillar E F, by turning the milled nut M; this nut is fixed to a pinion, that works in a toothed rack cut on one side of the pillar. By means of this pinion the stage may be gradually raised or depressed, and the object adjusted to the focus of the different lenses.

K is the slider-holder, which fits into a hole that is in the middle of the stage N I S; it is used to confine and guide either the motion of the sliders which contain the objects, or the glass tubes that are designed to confine small fishes, for viewing the circulation of the blood. The sliders and tubes are to be passed between the two upper plates.

L is a brass tube, in the upper part of which is fixed the condensing lens before spoken of; it screws into the wire arm a, which is placed in the hole I of the stage, with the glass underneath, and may be set at different distances from the object, according to its distance from the mirror or the candle.

O is the frame which holds the two reflecting mirrors, one of which is plane, the other concave. These mirrors may be moved in various directions, in order to reflect the light properly, by means of the pivots on which they move, in the semicircle Q, and the motion of the semicircle itself on the pin R; the concave mirror generally answers best in the day-time; the plane mirror combines better with the condensing lens in L, and a lamp or candle at night.

At S is a hole and slit for receiving either the nippers b, or the fish-pan c; when these are used, the slider-holder K must be removed.

T, a hole to receive the pin of the convex lens and illuminator d.

There are six magnifying lenses contained in a brass wheel screwed in a circular brass box P; this wheel is moveable about its center with the finger, and stops by a click when the magnifiers are each centrally under the body A B above, or the hole in the arm C D. They are marked from No. 1, to 6, and the proper number shewn in a small opening made in the side of the brass box. This wheel P screws into the arm C D, and may occasionally be taken off to admit of the silver speculum, or a single magnifier, hereafter to be described.

There is a small line cut on the edge of the arm C D, which must be brought to the right hand edge of its socket, in order to center the magnifier to the body and the stage.

By unscrewing the body A B, the single magnifiers in the wheel P being then only left, the instrument readily forms a single microscope.

A small pocket hand single or opake microscope may easily be extracted from this apparatus. When the body A B is screwed off, and the arm C D slipt away from its frame with the wheel of magnifiers, and the forceps, wire, and joint b applied to it, by a hole made in the arm for that purpose, as represented at V, it is then ready for the examination of any small object that may present itself in the garden, &c. and will be found very convenient whenever the whole instrument is not required.

LIST OF APPARATUS GENERALLY MADE TO THIS MICROSCOPE.

The wheel, with the magnifiers, P. Fig. 1.

The body of the microscope, A B.

The slider-holder, K.

The tube, with the condensing lens L, to be used by candle-light.

The pin and arm a, either for the above lens, or for the silver concave speculum e.

The silver concave speculum e, fitted to the arm above, and used common to all the magnifiers in the wheel and body A B, it is to reflect the light from the concave or plane mirror O below, upon the opake objects, then called the compound opake microscope.

A silver concave speculum f, with a single magnifier; it screws to the under part of the arm C D in room of the wheel of magnifiers, and forms then the single opake microscope.

A brass cone g, to place under the stage N I S, and serves to diminish the reflected light when necessary.

The jointed nippers b, fitted to the stage, to hold any small insect, or other opake object.

A cylinder of ivory h, to fix on the pointed end of the nippers, black on one side and white on the other, to make a contrast to the opake object used.

Six ivory sliders, i, each having four holes, and objects contained between two talcs confined together by brass circular wires. One of the sliders is usually sent without objects, to be supplied at pleasure. When used, they are placed between the perforated plates of the slider-holder K; where also is to be applied the brass frame slider k, containing in one brass piece four small concave glasses fixed; a narrow slip of glass slides over these, all within the frame; so that any very small living object, as a mite, &c., may be viewed with the proper security.

A set of glass tubes, l, three in number, to contain tadpoles, water newts, small frogs, eels, &c. which are curious objects for affording a fine view of the circulation of the blood, &c. They are also to be placed in the slider-holder K. There is a small hole at one end to admit air, the other end is to be stopped with cork, to contain the fluid and prevent the escape of the animal. A brass twisted wire is sent, to assist in the cleaning of these tubes.

A small ivory box, m, containing talcs and wires to supply the ivory sliders with, should any be lost or damaged.

A lens set in a brass cell, n, of such a focus as to view objects under a magnifying power sufficient for the applying them to the instrument for further inspection; hence it has been called the explorator. It may occasionally be screwed to the arm C D, and is then well adapted for viewing objects of the larger kind, or the whole of an insect, &c. before the observing of it in part under the regular magnifiers.

A concave, or a circular plane glass, o, for transparent objects, or animalcula in fluids, &c. it is fitted to the side, I, of the stage.

It is necessary to describe the lens and frame, d, noticed at page 95; it is either for converging the sun’s rays upon opake objects laid upon the stage, or for magnifying a flower, or other large objects applied to the stage, or on the nippers or point, b. By its pin and spring socket it is easily raised to any height, for the sun, candle, or the eye of the observer.

A brass insect box, h, consisting of a concave and plane glass that screw close together; by means of which a louse, flea, &c. may be secured, viewed alive, and retained for any time. It is applied to the hole I, of the stage, Fig. 1.

A pair of small brass forceps, q, by which any small object may be conveniently taken up or moved.

This microscope packs into a mahogany pyramidical shaped case, about seven inches square at its base, and fourteen inches in height. For its price, see the general list annexed to this work.

TO USE THIS MICROSCOPE.

It will be obvious to the reader from the preceding description that it must be put together as represented in the figure; that he has to place the slider-holder, K, to the stage, N I S, with one slider of objects; to reflect as strong a light as possible from the concave mirror, O, below, by turning it into the best position, and moving it upwards or downwards all the while he is looking down the body, A B. Then, for a distinct view of the object, to turn the pinion, M, in a slow and gentle manner. A small degree of practice will render the management very familiar.

For opake objects, the slider-holder, K, is to be removed; the silver speculum, e, screwed to the arm, a, and by its pin placed in the hole, I, of the stage, with the concave part downward above the stage; the glass, o, or the nippers, b, with ivory, h, placed at the stage: then the light reflected from the mirror, O, up to the speculum above, which will again reflect the light very strongly upon the object. Practice also in this case can make it easy to the beginner. The use of the rest of the apparatus has been sufficiently explained.

OF THE MOST IMPROVED COMPOUND MICROSCOPE, BEING UNIVERSAL IN ITS USES, AND FORMING THE SINGLE, COMPOUND, OPAKE, AND AQUATIC MICROSCOPES.

A person much accustomed to observations by the microscope, will readily discern the several advantages of this instrument over the preceding one. Besides its containing an additional quantity of useful apparatus, it is more commodious and complete for the management while observing, as it may instantly be placed in a vertical, oblique, or horizontal situation, turned laterally at the ease of the observer, and the objects viewed by the primary direct light, or reflected as usual, at pleasure. These particulars the following description will clearly shew. I shall not again so fully describe the same apparatus, as the reader must already understand their uses from the preceding references.

Fig. 2 is a representation of this instrument as placed up for use. A B is the compound body. The eye-glasses are contained in an inner tube that slides outwards or inwards, thus altering its distance from a glass at B, and thereby increasing or diminishing the magnifying power, when thought necessary. This body may be screwed on or off to the arm C D, as in the microscope just described; the arm C D may also be moved in any direction over the object. E F is the square stem or bar, on which is moved by the rack-work and pinion M, the stage N I S, to adjust a distinct view to any of the magnifiers, or apparatus used. V is a strong brass pillar with a joint-piece at top, connected to the stem E F; by means of this joint the position of the microscope is readily altered from a vertical to an oblique or horizontal one, as may be desired or found most easy and convenient to the observer, while sitting or standing; it will also enable him to view objects by direct unreflected light; for, when the stem, E F, is put into an horizontal position, the mirrors, O, R, may be drawn off and laid aside. Against or before the condensing lens, U, the common day-light or the light of a candle may then be directed.

At the stage N I S, is a sliding brass spring, N, serving to confine down slips of glass or large sliders, when objects placed thereon are intended to be viewed out of the horizontal position of the stage. A lens, U, called the condensing lens, fixed in a frame connected to a socket, is for condensing and modifying the rays of light reflected from the concave or plane mirror, O, below; it may be set to a proper distance by raising it up by two little screws, one of which is shewn at u. This lens is of considerable use by candle-light, as it serves to fill the whole body, A B, beautifully with light on the object. It is turned aside on a joint pin, when not in use. Six magnifiers are contained in the wheel at P, as in the former microscope. The mirrors, O, below may also be slid upwards or downwards on the stem, by pushing against the screws at r. Thus the stage, lens U, and mirrors below, being all in one axis of motion, admit the adjustment of the distinct view, light, &c. in the most accurate and pleasing manner. When the instrument is packed into its case, the feet, G G H, may all be folded together as one, and the body A B, screwed off, for the advantage of being portable. The body, as screwed off, leaves the instrument a single microscope.

THE GENERAL APPARATUS TO THIS MICROSCOPE IS AS FOLLOWS.

First, such as accompany the preceding microscope. The brass wheel with magnifiers, P, Fig. 2. The slider-holder, K. The brass pin and arm, a, for receiving the concave speculum, e, which is applied to the upper side of the stage, and used common to all the magnifiers. The silver concave speculum, f, with a magnifier set therein, used by itself in the arm C D. These two speculums form the instrument into what is called an OPAKE MICROSCOPE.

A brass cone, g, fitting the under side of the stage, N I S, to exclude superfluous light. The illuminator, or convex lens, d, Fig. 1, fitted to T of the stage. The jointed nippers, b, fitted to the stage, and either on the point or nippers to hold any small insect, or other opake object. An ivory black and white piece, h, is also fitted to the point to contrast the colour of any object laid thereon; the light upon this is reflected from the silver concaves placed above, which reflect the light downwards received from the mirrors at O. Six ivory sliders as shewn at i, containing a selection of objects, placed between Muscovy talc, and fastened by spring wires; and a brass frame slider, k: all for the stage, K, when in use. A set of glass tubes for fish or liquids, l, to be filled with water and stopped with cork, for the slider-holder K. A pan, c, for fish or frogs, fitted to the stage at S. A small ivory box, m, with spare talcs and wires. The explorator, n, a lens set in a brass cell, for viewing the larger sort of objects either by the hand, or from the arm C D, Fig. 2. A plane glass, o, and a concave ditto, s, both fitted to the hole of the stage, N I S, for viewing fluids, and confining the animalcula, &c. between them, and so forming what is called the AQUATIC MICROSCOPE.

A brass box, p, with a concave and plane glass, for insects and other objects, fitted to the stage N I S, when they are to be examined by the instrument. A pair of brass forceps, q, to take or hold any object by. A camel hair brush, t.

ADDITIONAL APPARATUS TO THIS BEST MICROSCOPE.

Three large wood sliders, as shewn at X, with talcs and wires, for the larger sort of wings of flies, and other objects which are too large for the small ivory sliders, i; they are to be placed in the slider-holder K, when on the stage N I S, and the objects to be magnified either by the magnifiers in the wheel P, or the lens shewn at n, screwed on the arm C D. A brass cell, y, with a very small globule or lens, or an extraordinary great magnifier, usually about the 30th or 40th of an inch focus; it is to be screwed into the arm C D, when the wheel, P, is first unscrewed away. It is for the purpose of viewing extreme minute objects, which may be so small as to elude the power of the magnifiers in the wheel, P.

A moveable stage, W, which by the pin, a, is applied to the hole, S, of the stage Fig. 2, and thereby has an horizontal motion under the whole field of view, without disturbing any other part of the instrument. To the large hole of this stage are fitted a deep concave glass, r, and the concave and plane glasses, s and o; and to the small holes, x x, a black and white piece of ivory, w, for opake objects, and a concave and plane glass similar to o and s. An extra concave silver speculum with a less magnifier than the other, as shewn at f, used for the larger kind of opake objects, like the other, fitted to the arm C D, and used instead of the magnifiers in the wheel, P.

Rack-work is sometimes cut in the arm C D, to turn the pinion above, so as to move the magnifiers in a linear direction over the objects in the most accurate degree; and also the stage N I S jointed, to turn by a screw and teeth in an horizontal direction at right angles to the above, thereby rendering a slow and accurate motion, perfectly suitable to the various positions of any living animal under examination.

Six or more larger ivory sliders, with cuttings of different woods, &c. are also frequently added; but as these enhance the expense, and may be extended to the desire of the purchaser, his choice, and not my description here, will determine the extent of the apparatus to the microscope. When packed up into its mahogany, or black shagreen case, the outside dimensions are about twelve inches and an half long, nine inches broad, and three inches three-quarters deep.

A microscope from this plan is frequently made of smaller dimensions, for the convenience of persons who frequently travel, and is contained in a fish-skin case about seven inches long, four inches and an half broad, and two inches deep, and is the most complete instrument of the sort.

TO USE THIS MICROSCOPE.

As in the former one, place the slider-holder K, with a slider of objects in it, in the stage N I S; move the arm C D, in its socket, so that a mark on the side is brought to the edge of the socket; then turn the arm till the magnifier is directly central over the object; look down the tube A B, and during that time, reflect the light strongly and clearly up into it from the mirror O below; and then, while you are looking through the body, gently turn the pinion at M to the right or left, till you see the object magnified in the most distinct and well-defined manner. Attending properly to this mode is the only care necessary to use any microscope whatsoever; and for want of doing which, many a beginner finds a difficulty in using properly his instrument. For price, see the list at the end.

For opake objects, you take away the slider-holder, K; place on the stage either the concave glass, s, or the nippers, b; screw the concave speculum, e, to the arm, a, which place on the stage with the arm in the hole, I. The light is now to be reflected into this concave dish from one of the mirrors, O, below, and it will thus be strongly condensed upon the object. With this concave speculum any of the magnifiers in the wheel, P, may be used. When the single silver concave, f, is used, it is screwed to the arm C D, and the one, e, and arm, a, are not then applied.

For further directions for the management of microscopes, the light, &c. see Chap. IV. p. 129, and sequel.

A DESCRIPTION OF CULPEPER’S, OR THE COMMON THREE-PILLARED MICROSCOPE. Plate IV. Fig. 3.

The only recommendations of this original instrument are, its simple construction and lowness of price. It gives a pleasing view of the object. It is precluded by its form from some of the advantages of the two foregoing instruments, because both the stage and the mirror are confined. This microscope consists of a large exterior brass body, A B, supported on three brass scrolls, which are fixed to the stage F; the stage is supported by three larger scrolls that are screwed to the mahogany pedestal G H. There is a drawer in the pedestal which holds the apparatus. The concave mirror, I, is fitted to a socket in the center of the pedestal. The lower part, B, of the body forms an exterior tube, into which the upper part of the body, C, slides, and may be moved up or down by the hand, so as to bring the magnifiers which are screwed on at D, nearer to, or further from the object.

A LIST OF THE APPARATUS TO CULPEPER’S MICROSCOPE.

Five magnifiers, each fitted in a brass cell; one of these is seen screwed on at D. Six ivory sliders, k, five of them with objects; and a small ivory box, m, containing some spare talcs, and wires for them. A brass tube, N, to hold the concave speculum. A brass box, M, for the same speculum. A fish-pan, c. A set of glass tubes, b. A flat and a concave glass, both fitted to the stage. A brass cone, g, to exclude superfluous light; it applies at the under side of the stage, F. A brass box, p, with plane and concave glasses for living objects. A pair of forceps, q. A steel wire, b, with a pair of nippers at one end, a point at the other, and a small ivory cylinder, h, to fit on the pointed end of the nippers. A convex lens, E, moveable in a brass semicircle; this is affixed to a long brass pin, which fits into a hole, F, on the stage. The uses of the above apparatus have been sufficiently described in the preceding pages.

TO USE THIS MICROSCOPE.

Screw one of the five cells, which contains a magnifying lens, to the end, D, of the body; place the slider i or k, with the objects, between the plates of the slider-holder, K. Then, to attain distinct vision and a pleasing view of the object, adjust the sliding body to the focus of the lens you are using, by moving the upper part, C, gently up and down while you are looking at the object, and regulate the light by the concave mirror, I, below. The image of the objects in this microscope is seen in a field of view of about six inches in diameter; but, in the improved ones before described, it is from about twelve to fifteen inches.

For opake objects, two additional pieces must be used; the first is a cylindrical tube of brass, represented at N, which fits on the cylindrical snout above D of the body: the second piece is the concave speculum, L; this is to be screwed to the lower end of the aforesaid tube. The upper edge of this tube should be made to coincide with the line which has the same number affixed to it as the magnifier you are using; that is, if you are making use of the magnifier marked 5, slide the tube to the circular line on the tube above D, that is marked also with No. 5.

The slider-holder, K, should be removed when you are going to view opake objects, and a plane glass should be placed on the stage in its stead to receive the object; or it may be placed on the nippers, b, the pin of which fits into the hole in the stage.

A DESCRIPTION OF MARTIN’S IMPROVED SOLAR MICROSCOPE, WHICH IS CONSTRUCTED TO EXHIBIT TRANSPARENT AND OPAKE OBJECTS. Plate V.

The solar microscope is generally supposed to afford the most entertainment, on account of the wonderful extent of its magnifying power, and the ease with which several persons may view each single object at the same time. The use of it was, however, confined for many years only to transparent objects. About the year 1774, Mr. B. Martin so far improved this instrument, as to render it applicable to opake, as well as to transparent objects, exhibiting the magnified image of either kind on a large screen. Treating of it himself, he says[34], “With this instrument all opake objects, whether of the animal, vegetable, or mineral kingdom, may be exhibited in great perfection, in all their native beauty; the lights and shades, the prominences and cavities, and all the varieties of different hues, teints, and colours, heightened by the reflection of the solar rays condensed upon them.” From its enlarged dimensions, transparent objects are also shewn with greater perfection than in the common solar microscope.

[34] Description and Use of an Opake Solar Microscope. 8vo. 1774.

Plate V. Fig. 1, represents the solar opake microscope, placed together for exhibiting opake objects.

Fig. 2, is that part called the single tooth and pinion microscope, which is used for shewing transparent objects; the cylindrical tube, Y, thereof, being made to fit into the tube E F, Fig. 1. It may be occasionally used as a hand single, or Wilson’s microscope, and for which purpose, the handle, c, is fitted by a screw to the body at g, and the tube, Y, screwed away.

Fig. 3, the slider which contains the six magnifiers; it fits into a dove-tail under P, Fig. 2, at the upper part of the microscope.

Fig. 4 represents a brass dove-tail slider, containing a small lens: it is called a condenser. There are three in number, marked 1 and 2, &c. corresponding to the number of the magnifiers used: they serve to condense the sun’s rays strongly upon the object, and enlarge the circle of light. They slide in at h, Fig. 2.

A B C D E F, Fig. 1, represents the body of the solar microscope; one part thereof, A B C D, is conical, the other, C D E F, is cylindrical. The cylindrical part receives the tube, G, of the opake object box, or the tube, Y, of the single microscope, Fig. 2. At the large end, A B, of the conical part there is a convex lens to receive the rays from the mirror, and refract them convergingly into the box, H I K L.

N O P is a brass frame which is fixed to the moveable circular plate, a b c; in this frame there is a plane mirror, to reflect the solar rays through the afore-mentioned lens. This mirror may be moved into the proper positions for reflecting the solar rays, by means of rack-work turned by the nuts Q and R. By the nut Q, it may be moved from right to left; it maybe elevated or depressed by the nut, R. d e, two screws to fasten the microscope to a window-shutter, or a board fitted entirely before the window.

The box for opake objects is represented as open at H I K L; it contains a plane mirror, M, for reflecting the light that it receives from the large lens to the object, and thereby illuminating it; S is a screw to adjust this mirror to its proper angle for reflecting the light. V X, two tubes of brass, one sliding within the other, the exterior one in the box, H I K L; these carry two magnifying lenses: the interior tube is sometimes taken out, and the exterior one is then used by itself. Part of this tube may be seen in the plate as within the box, H I K L.

At H, is a brass plate, the back part of which is fixed to a tube, h, containing a spiral wire, which keeps the plate always bearing against the side, H, of the brass box H I K L. The sliders, with the opake objects, Fig. 5, pass between this plate and the side of the box; to apply which, the plate is to be drawn back by means of the nut, g. k i, a door to one side of the opake box, to be opened when adjusting the mirror, M.

The foregoing pieces constitute the several parts necessary for viewing opake objects. We shall now proceed to describe the single microscope, which is used for transparent objects; but, in order to examine these, the box, H I K L, must be first removed, and in its place we must insert the tube, Y, of the single microscope, Fig. 2, now to be explained.

Fig. 2 represents a large tooth and pinion microscope; at m, within the body of this microscope, are two thin plates that are to be separated, in order to let the ivory sliders, Fig. 7, pass between them; they are pressed together by a spiral spring, which bears up the under plate, and forces it against the upper one. The slider, Fig. 3, that contains the magnifiers, fits into a hole at n; any of the magnifiers may be placed before the object, by moving the aforesaid slider: when the magnifier is at the center of the hole P, a small spring falls into one of the notches which is on the side of the slider, Fig. 3. At h, slides a condenser, Fig. 4, for condensing the sun’s rays, and enlarging the field of view on the screen: the number must correspond with that of the magnifier used. This microscope is adjusted to the focus, while exhibiting the object, by turning the milled nut O.

APPARATUS TO THE OPAKE SOLAR MICROSCOPE.

The mirror O P, Fig. 1, and square plate, and the tubular body of the microscope, A F. The opake box and its tube, I K G. The tooth and pinion or single microscope, Fig. 2. The slider of magnifiers, Fig. 3. The megalascope magnifier, Fig. 6, fitted to P of Fig. 2. Six ivory sliders with transparent objects, Fig. 7. Twelve wood sliders with opake objects, and a brass frame to hold them, Fig. 5. A brass square-formed slider case, Fig. 8, to hold any animal, piece of ore, or other opake object, and is to be placed like the other slider at H, Fig. 1. A pair of nippers and point, Fig. 9, the pin, a, of which fits into the hole of the slider, Fig. 4, and holds before the magnifiers at P, Fig. 2, any small fly or other complete object to be magnified. A four-glass slider in a brass frame, Fig. 10, for any animalcula, &c. to be placed between the plates at m, Fig. 2. A set of glass fish tubes, Fig. 11. A pair of forceps, Fig. 12. Two brass nuts for the window-shutter or board, Fig. 13; and the two brass fastening screws, d e, Fig. 1, which may be either used with or without the above two nuts.

The figures on the plate are about half the original size, and the apparatus now made by Messrs. Jones packs into a case thirteen inches long, nine inches broad, and four inches deep. For price, see the list at the end.

TO USE THE SOLAR MICROSCOPE.

Make a round hole in a window-shutter or window-board, that is opposite to the meridian sun, or as nearly so as possible, a little larger than the circle a b c; pass the mirror, N O P, through this hole, and apply the square plate to the shutter; then mark with a pencil the places which correspond to the two holes through which the screws are to pass; take away the microscope, and bore two holes at the marked places, large enough to admit the milled screws, d e, to pass through them. These screws are to pass from the outside of the shutter, to go through it, and being then screwed into their respective holes in the square plate, they will, when screwed home, hold it fast against the inside of the shutter, and thus support the microscope.

Another way, and perhaps more convenient, is to previously screw the two brass nuts, Fig. 13, to the shutter or window-board, at the inside at a suitable distance, to receive the two milled screws; these nuts will always be ready for use, and the operator may in a minute, within his room, fasten the plate, a b c, to the shutter by the two milled screws, being placed contrarywise.

Screw the conical tube, A B C D, to the circle, a b c, and then slide the tube, G, of the opake box into the cylindrical part, C D E F, of the body, if opake objects are to be examined; but if transparent objects are intended to be shewn, then place the tube Y, Fig. 2, within the tube C D E F. The room is to be darkened as much as possible, that no light may enter but what passes through the body of the microscope; for, on this circumstance, together with the brightness of the sun, the perfection and distinctness of the image in a great measure depend.

We shall first consider the microscope as going TO BE USED FOR OPAKE OBJECTS. Adjust the mirror, N O P, so as to receive the solar rays, by means of the two finger-screws or nuts, Q, R; the first, Q, turns the mirror to the right or left; the second, R, raises or depresses it: this you are to do, till you have reflected the sun’s light through the lens at A B, strongly upon a white-paper screen or cloth, from four to eight feet square (about the latter dimensions for transparent objects) placed from about five to eight feet distance from the window, and formed thereon a round spot of light: a white wainscot or wall at a suitable distance answers very well. An unexperienced observer will find it more convenient to obtain the light by first forming this spot, before he puts on either the opake box, or the tooth and pinion microscope, Fig. 2.

Now apply the opake box, and place the object between the plates at H; open the door, k i, and adjust the mirror, M, till you see you have illuminated the object strongly. If you cannot effect this by the screw S, you must move the screws Q, R, in order to get the light reflected strongly from the mirror, N O P, on the mirror M; without which the latter cannot illuminate the object. The object being strongly illuminated, shut the door, k i, and a distinct view of the object will soon be obtained on your screen, by adjusting the tubes V X, with the magnifiers, which is effected by moving them backwards or forwards.

A perfectly round spot of light cannot always be procured in northern latitudes, the altitude of the sun being often too low; neither can it be obtained when the sun is directly perpendicular to the front of the room. As the sun is continually changing its place, it will be necessary, in order to keep his rays full upon the object, to keep them continually directed through the axis of the instrument, by turning the two screws Q and R.

To view transparent objects, remove the opake box, and insert the tube, Y, of Fig. 2, in its place; put the slider, Fig. 3, into its place at n, a condenser, Fig. 4, at h, and the slider with the objects between the plates at m; then adjust the mirror, N O P, as before directed, by the screws, Q, R, so that the light may pass through the object; regulate the focus of the magnifier by the pinion, O. The most pleasing magnifiers in use are the fourth and fifth. The size of the object is generally from four to eight feet, and may be increased or diminished by altering the distance of the screen from the microscope; five or six feet is a convenient distance.

The effect by this sort of microscope is stupendous, and never fails to excite wonder in an observer at the first view, in seeing a flea, &c. augmented in appearance to SEVEN, EIGHT, or even TEN FEET in length, with all its colours, motions, and animal functions, distinctly and beautifully exhibited.

To examine transparent objects of a larger size, or to render the instrument what is usually called a megalascope, take out the slider, Fig. 3, from its place at n; screw the cell and lens, Fig. 6, into the hole at P, Fig. 2; remove the glass which is placed at h, and regulate the light and focus agreeable to the foregoing directions.

At C D, is placed a lens for increasing the density of the rays, for the purpose of burning or melting any fusible substance; this lens must be removed in most cases, lest the objects should be burnt. The intensity of the light is also varied by moving the tube G, and Fig. 2, Y, inwards or outwards.

DESCRIPTION OF THE TRANSPARENT SOLAR MICROSCOPE AND APPARATUS. Plate VI. Fig. 4, to 14.

The foregoing description will, in great part, answer for this microscope; but, the dimensions, apparatus, &c. varying in a small degree from the preceding, a distinct description here, may be acceptable to those, who possess this sort of microscope only.

A B C D, Fig. 4, represents the body of the microscope, consisting of two brass tubes. E F is the end of the inner moveable tube; e f, that of the single tooth and pinion microscope. Fig. 5, screws into the end of this inner tube; at the end, A B, of the external tube there is a convex lens, to receive the sun’s rays from the mirror, K L, and to condense them on the object; the end, A B, screws into the circular plate, G H I. This part may also be used as a single microscope, and may have at m the handle, c, screwed to it. K L, a long frame fixed to the moveable circular plate, with a plane mirror, to reflect the rays of the sun on the lens at A B. An endless worm or screw, which is cut on the lower part of the nut, M, works in a small wheel which is fixed to the frame, K L, so that by turning the nut, the frame, K L, is moved up or down: the nut, N, moves the mirror to the right or left. O, P, two screws to fasten the square plate to the window-shutter.

Fig. 5, the single microscope; e f, the end which screws on to the part, E F, Fig. 4, of the internal tube of the body; q, the dove-tailed slit for receiving the slider, Fig. 8; g, the hole in which the megalascope magnifier, Fig. 6, is to be screwed, when the slider, Fig. 8, is removed. At h, are the moveable plates, between which the object sliders are placed; under the lowermost of these, the lens represented at Fig. 11 is to be placed, when the magnifiers in the slider, Fig. 8, are to be used, a k is a small piece of rack-work, which is moved backwards and forwards by the pinion fixed to the milled nut, b; by the gradual motion of this rack, the objects are adjusted to the foci of the different lenses. Fig. 8 is a brass slider, with six lenses, or magnifying glasses; it is to be inserted into the hole at q; either of the magnifiers may be placed before the object, by sliding it one way or the other: you may perceive when the glass is in the center of the eye-hole by a small spring acting upon a notch which is made on the side of the slider opposite to each lens.

APPARATUS BELONGING TO THIS SOLAR MICROSCOPE.

Square plate and mirror. The body, A D, consisting of two tubes, one within the other. The single microscope, Fig. 5. The megalascope lens, Fig. 6. The slider, Fig. 8, with six lenses. The two screws O, P. Six ivory sliders and a talc box, Fig. 7 and 13. Some glass tubes, Fig. 9. A slider or brass case, Fig. 10, containing a plane piece of glass, and a brass slider with holes, into which are cemented small concave glasses, designed for confining minute insects between the plane and concave glasses, which are thus preserved from being crushed, or from moving out of the field of view. Three condensing lenses to enlarge the field of view, such as Fig. 11, that are fitted to the hole, l, of Fig. 5. Their numbers correspond with the numbers used. Fig. 12, two brass nuts for the window-shutter or board, to receive the two screws, O and P.

To use the transparent solar microscope.

Fasten the square plate against the inside of a window-shutter, by the two screws O, P, which are to go from the outside of the window-shutter through it, and then be screwed into their respective holes in the square plate at G H I. The mirror is to be on the outside of the shutter, passing through a hole made for that purpose. Darken the room; then place a screen at about six or eight feet distance from the window, the farther it is from it the larger is the image: now move the mirror, K L, by the two nuts M N, till the sun’s rays come through the instrument in an horizontal direction to the screen, forming a round spot thereon; screw the microscope, Fig. 5, into its place E F; put the slider with the lenses, Fig. 8, at q, Fig. 5, and the object slider between the plates at h; adjust the object to the focus of the magnifying lens by the screw b, till the object appears distinct and clear on the screen. By moving the internal tube of the body, the object may be placed at different distances from the lens which is fixed at A B, so as to be sufficiently illuminated, and not burnt by the solar rays. If the screws O, P, are to pass inside the room, the two nuts, Fig. 12, must be previously fixed.

THE SCREW BARREL, OR WILSON’S SINGLE POCKET MICROSCOPE. Plate II. B. Fig. 1 and 2.

This microscope of Mr. Wilson’s is an invention of many years standing, and was in some measure laid aside, till Dr. LieberkÜhn introduced the solar apparatus to which he applied it, there being no other instrument at that time which would answer his purpose so well; it is much esteemed in particular cases. The body of the microscope is represented at A B, Fig. 1, and is made either of silver, brass, or ivory. C C is a long fine-threaded male screw, that turns into the body of the microscope. D, a convex glass at the end of the said screw, on which may be placed, as occasion requires, one of the two concave apertures of thin brass to cover the said glass, and thereby diminish the aperture when the greatest magnifiers are used. E, three thin plates of brass within the body of the microscope, one whereof is bent to an arched cavity for the reception of a tube of glass. F, a piece of wood or brass, curved in the manner of the said plate, and fastened thereto. G, the other end of the microscope, where a female screw is adapted to receive the different magnifiers. H, a spiral spring of steel, between the said end, G, and the plates of brass, E, intended to keep the plates in a due position, and counteract against the long screw, C. I, a small ivory handle. To this microscope belong seven different magnifying glasses, six of which are set in cells, as in Fig. K, and are marked from 1, to 6: the lowest numbers to the greatest magnifiers. L is the seventh magnifier, set in the manner of a little barrel, to be held in the hand for viewing any large object. M is an ivory slider with the objects. Six of these, and one of brass, are usually sold with this microscope. There is also a brass slider not shewn in the figure, to confine any small object, that it may be viewed without crashing or destroying it. N, a pair of forceps, or pliers, for the taking up of insects or other objects, and applying them to the sliders or glasses. O, a camel hair brush, to take up and examine a small drop of liquid, brush the dust away, &c. P is a glass tube to confine living objects, such as frogs, fishes, &c.

When you view an object, push the ivory slider, in which the said object is placed, between the two flat brass plates, observing always to put that side of the slider, where the brass rings are, farthest from the eye; then screw in the magnifying glass you intend to use at the end of the instrument G, and looking through it against the light, turn the long screw, C C, till your object is brought to appear distinct, or to the true focal distance. To examine any object accurately, view it first through a magnifier that will shew the whole at once, and afterwards inspect the several parts more particularly with one of the greatest magnifiers; for thus you will gain a true idea of the whole, and all its parts: and, though the greatest magnifiers can shew but a minute portion of any object at once, such as the claw of a flea, the horn of a louse, &c. yet by gently moving the slider that contains your object, the eye will gradually see the whole; and if any part should be out of the focal distance, the screw, C C, will easily bring it to the true focus. As objects must be brought very near the glass, when the greatest magnifiers are used, be particularly careful not to rub the slider against the glasses as you move it in or out. A few turns of the screw, C C, will easily obviate this.

DESCRIPTION OF A SCROLL FOR FIXING WILSON’S POCKET MICROSCOPE, AND A MIRROR FOR REFLECTING LIGHT INTO IT.

A B C, Fig. 2, is a brass scroll, which, for the better conveniency of carriage, is made to unscrew into three parts, and may be put into the drawer upon which it stands, with its reflecting mirror D, and Wilson’s pocket microscope, G. The upper part of the scroll is taken off at B, by unscrewing half a turn of the screw; then, if lifted up, it will come out of the socket. The lower part unscrews at C, and the base at E. The mirror lifts out at F, which, with the scroll, lies in one partition of the box.

To apply this scroll for use, fix the body of the microscope to the top thereof by the screw, A, as in Fig. 2, by screwing it in the same hole as the ivory handle was applied to before. The brass or ivory slider being fixed as before described, and the microscope placed in a perpendicular position, move the mirror, D, in such a manner as to reflect the light of the sky, of the sun, or a candle, directly upwards through the microscope; by which means the object will be most conveniently viewed. It is further useful for viewing opake objects, by screwing the arm, Q R, Fig. 1, into the body of the microscope at G; then screwing into the round hole, R, that magnifier which you think will best suit your object, and putting the concave speculum, S, on the outside of the ring, R, you will observe in the body of the microscope, between the wood or brass, F, and the end of the male screw, C C, a small hole, u, through which slides the long wire, T, which has a point at one end, and forceps at the other, that may be used occasionally as your objects require. When you have fixed this, and your object on it, turn the arm, R, till the magnifier is brought over the object; it may be then adjusted to the true focus, by turning the screw, as before. It must also be brought exactly over the speculum, by turning the upper part of the scroll to one side, till your object and the two specula are in one line, as will be found by trial; and then fix it by the screw, B, at which time the upper surface of the object will be enlightened by the light reflected from the mirror, D, to the concave speculum.

DESCRIPTION OF A SMALL MICROSCOPE FOR OPAKE OBJECTS. Plate II. B. Fig. 3 and 4.

A, Fig. 3, is a fixed arm, through which passes a screw, B, the other end is fastened to the moveable arm, C. D, a nut fitted to the said screw, which, when turned, will either separate or bring together the two arms, A C. E, a steel spring, that separates the two sides when the nut is unscrewed. F, a piece of brass turning round in a spring socket, moving on a rivet, in which moves a steel wire pointed at the end G, and the other end a pair of pliers, H: these are either to thrust into, or take up and hold any object, and may be turned round as required. I, a ring of brass, with a female screw fixed on an upright piece of the same metal, turning on a rivet, that it may be set at a due distance when the least magnifiers are used, and is adapted to the screws of all the magnifiers.

Fig. 4, K, a concave speculum of polished silver, in the center of which a lens is placed. On the back of this speculum a male screw, L, is made to fit the brass ring I, Fig. 3. Four of these specula of different concavities, with four glasses of different magnifying powers, as the objects may require. The greatest magnifiers have the least apertures. M, a round object plate, one side white and the other black, intended to render objects the more visible, by placing them, if black, upon the white, and if white, on the black side. A steel spring, N, turns down on each side, to secure any object; from the object plate there is a hollow pipe, to screw it on the needle’s point G, Fig. 3. O, a small box of brass, with a glass on each side, to confine any living object in order to examine it, having a pipe to screw upon the end of the needle at G. P, an ivory handle. Q, a pair of pliers to take up any object. R, a soft hair brush.

To view any object, screw the speculum, with the magnifier you intend to use, into the brass ring, I; place your object either on the needle G, in the pliers H, on the object plate M, or in the brass hollow box O, as may be most convenient; then holding up your instrument by the handle P, look against the light through the magnifying lens, and by means of the nut, D, together with moving of the needle at its lower end, the object may be turned about, raised or depressed, brought nearer the glass, or put farther from it, till you have the true focal distance, and the light be seen reflected from the speculum strongly upon the object.[35]

[35] Opake microscopes are now constructed more elegantly and simply. The chief merit of Wilson’s microscope appears, in being particularly adapted to minute objects, and these principally of the transparent kind; the barrel form is useful for excluding adventitious light. Excepting these peculiarities, its general utility is considered far short of the universal pocket microscope hereafter to be described. Edit.

OF ELLIS’S SINGLE OR AQUATIC MICROSCOPE. Plate VII. B.

This instrument takes its name from Mr. John Ellis, author of “An Essay towards a Natural History of Corallines,” and of the “Natural History of many curious and uncommon Zoophytes.” By this instrument he was enabled to explain many singularities in the oeconomy and construction of these wonderful productions of nature. To the practical botanist this instrument is recommended by the respectable authority of Mr. Curtis, author of the Flora Londinensis, a work which does credit to the author and the nation. This microscope is simple in its construction, easy in its use, and very portable; these advantages, as well as some others which it also has over other portable microscopes, have accelerated the sale thereof, and caused it to be very much adopted.

DESCRIPTION OF THE VARIOUS PARTS OF THE MICROSCOPE.

K, the box which contains the whole apparatus; it is generally made of fish-skin; on the top of the box there is a female screw, for receiving the screw which is at the bottom of the brass pillar A, and which is to be screwed on the top of the box, K. D, a brass pin which fits into the pillar; on the top of this pin is a hollow socket to receive the arm which carries the magnifiers; the pin is to be moved up and down, in order to adjust the lenses to their focal or proper distance from the object.

In the representation of this microscope, Plate VII. B. Fig. 1, the pin, D, is delineated as passing through a socket at one side of the pillar, A; it is now usual to make it pass down a hole bored through the middle of the pillar.

E, the bar which carries the magnifying lens; it fits into the socket, X, which is at the top of the pillar, D. This arm may be moved backwards and forwards in the socket X, and sidewise by the pin, D; so that the magnifier, which is screwed into the ring at the end, E, of this bar, may be easily made to traverse over any part of the object lying on the stage or plate B. F is a polished silver speculum, with a magnifying lens placed at the center thereof, which is perforated for this purpose. The silver speculum screws into the arm E, as at F. G, another speculum of a different concavity from the former, with its lens. H, the brass semicircle which supports the mirror, I; the pin, R, affixed to the semicircle, H, passes through the hole which is towards the bottom of the pillar, A. B, the stage or the plane on which the objects are to be placed; it fits into a small dove-tailed arm which is at the upper end of the pillar, A. C, a plane glass, with a small piece of black silk stuck on it; this glass is fitted to a groove made in the stage, B. M, a deep concave glass, to be laid occasionally on the stage instead of the plane glass, C. L, a pair of nippers; these are fixed to the hole of the stage, a, by the pin K; the steel wire of these nippers slides backwards and forwards in the socket, and this socket is moveable upwards and downwards by means of the joint, so that the position of the object may be varied at pleasure. The object may be fixed in the nippers, stuck on the point, or affixed by a little gum-water, &c. to the ivory cylinder, N. O, a small pair of brass forceps to take up minute objects by. P, a brush to clean the glasses.

To use this microscope; begin by screwing the pillar, A, to the cover thereof; pass the pin, R, of the semicircle which carries the mirror through the hole that is near the bottom of the pillar, A; push the stage into the dove-tail at B; slide the pin into the pillar, then pass the bar, E, through the socket, X, which is at the top of the pin D, and screw one of the magnifying lenses into the ring at F.

Now place the object either on the stage, or in the nippers L, and in such a manner, that it may be as nearly as possible over the center of the stage; bring the speculum, F, over the part you mean to observe; then get as much light on the speculum as you can, by means of the mirror, I; the light received on the speculum is reflected by it on the object. The distance of the lens, F, from the object is regulated by moving the pin, D, up and down, until a distinct view of it is obtained. The rule usually observed is, to place the lens beyond its focal distance from the object, and then gradually slide it down, till the object appears sharp and well defined. The adjustment of the lenses to their foci, and the distribution of the light on the object, are what require the most attention.

These microscopes are sometimes fitted up with a rack and pinion to the pillar A, and pin D, for the more ready adjustment of the glasses to their proper foci.

DESCRIPTION OF LYONET’S ANATOMICAL MICROSCOPE. Plate VI. Fig. 3.

Fig. 3 represents the instrument with which M. Lyonet made his microscopical and wonderful dissection of the chenille de saule or caterpillar of the goat moth,[36] of which a specimen is given in Plate XII. Fig. 1, &c. of this work. This portable instrument needs no further recommendation. By it, other observers may be enabled to dissect insects in general with the same accuracy as M. Lyonet, and thus advance the knowledge of comparative anatomy, by which alone the characteristic, nature, and rank of animals, can be truly ascertained.

[36] PhalÆna cossus. Linn. 63.

A B is the anatomical table, which is supported by the pillar O N; this is screwed on the mahogany foot, D C. The table A B, is prevented from turning round by means of two steady pins; in this table or board there is a hole, G, which is exactly over the center of the mirror, F E, that is to reflect the light on the object; the hole, G, is, designed to receive a flat or a concave glass, on which the objects are to be placed that you design to examine or dissect. R X Z is an arm formed of several balls and sockets, by which means it may be moved in every possible position; it is fixed to the board by means of the screw, H; the last arm, I Z, has a female screw, into which a magnifier may be screwed, as at Z. By means of the screw, H, a small motion may be occasionally given to the arm I Z, for adjusting the lens with accuracy to its focal distance from the object. Another chain of balls is sometimes used, carrying a lens to throw light upon the object; the mirror is also so mounted, as to be taken from its place at K, and fitted on a clamp, by which it may be fixed to any part of the table, A B.

To use the dissecting table.

Let the operator sit with his left side near a light window; the instrument being placed on a firm table, the side, D L, towards his breast, the observations should be made with the left eye: this position is well adapted for observing, drawing, or writing. In dissecting, the two elbows are to be supported by the table on which the instrument rests, the hands resting against the board, A B, in order to give it greater stability, as a small shake, though imperceptible to the naked eye, is very visible in the microscope; the dissecting instruments are to be held one in each hand, between the thumb and two fore-fingers. Farther directions are given on the mode of dissecting small objects in the following chapter.

DR. WITHERING’S BOTANICAL MICROSCOPE. Plate VI. Fig. 1.

This small instrument consists of three brass parallel plates, A, B, C; two wires, D and E, are rivetted into the upper and lower plate; the middle plate or stage is moveable on the aforesaid wires, by two little sockets which are fixed to it. The two upper plates each contain a magnifying lens, but of different powers; one of these confines and keeps in their places the fine point F, the forceps G, and the small knife H.

To use this instrument, unscrew the upper lens, and take out the point, the knife, and the forceps; then screw the lens on again, place the object on the stage, and then move it up or down till you have gained a distinct view of the object, as one lens is made of a shorter focus than the other; and spare lenses of a still deeper focus are sometimes added. The principal merit of this microscope is its simplicity.

THE POCKET BOTANICAL AND UNIVERSAL MICROSCOPE.

This pocket instrument is represented at Plate VI. Fig. 2. It is by most naturalists deemed preferable to Dr. Withering’s, being equally simple, more extensive in its application, and the stage unincumbered; though that of M. Lyonet seems better adapted than either to the purposes of dissection only.

A B, a small arm, carrying three magnifiers, two fixed to the upper part, as at B, the other to the lower part of the arm, at C; these may be used separately or combined together, by which you have seven powers. The arm, A B, is supported by the square pillar I K, the lower end of which fits into the socket, E, of the foot, F G; the stage, D L, is made to slide up and down the square pillar. H, a mirror for reflecting light on the object.

To use this microscope, place the object on the stage, L, reflect the light on it from the mirror H, and regulate it to the focus, by moving the stage nearer to or further from the lenses at B C. The ivory sliders pass under the stage, L; other objects may be fixed in the nippers, M N, and then brought under the magnifiers; or they may be laid on one of the glasses fitted to the stage. The apparatus to this instrument consists of three ivory sliders, a pair of nippers, a pair of forceps, a flat glass, and a concave ditto, all fitted to the stage, L. By taking out the pin, M, the pillar, I K, may be turned half round, and the foot, F G, made to answer as an handle.[37]

[37] An adjusting screw, Fig. 13*, to move the stage, with other additions, are made by Messrs. Jones; and which then, in my opinion, constitute the most complete pocket microscope hitherto made; for the particulars of which, I refer the reader to their printed description. Fig. 14, represents the common flower or insect microscope. There are two lenses, a and b, that are used separately or conjointly. Edit.

BOTANICAL MAGNIFIERS.

Since botany has been cultivated with so much ardor, it has been found necessary to contrive some very portable instrument, by which the botanist might investigate the object of his pursuits as it rises before him. Plate VIII. Fig. 7 and 8, represent two of the most convenient sort.

In the tortoiseshell case, Fig. 7, three lenses are contained, d, e, f, of different foci, which are all made to turn into the case, and may be used combined or separately. The three lenses in themselves afford three different magnifying powers; by combining two and two, we make three more; and the three together make, a seventh magnifying power. When the three lenses are used together, it is best to turn them into the case, and look through the hole, for more distinctness, and the exclusion of superfluous light. In the case, Fig. 8, are also three lenses, g, h, i, of different magnifying powers, that all turn up, and shut into the case; but these are not capable of combination.

DESCRIPTION OF A PORTABLE MICROSCOPE AND TELESCOPE. Plate VIII. Fig. 1, to 6.

The telescope is one of those which are composed of several sliding drawers or tubes, for the convenience of being put into the pocket; the sliding tubes are made of thin brass, the outside tube of mahogany. The sliding tubes are contrived to stop, when drawn out to a proper length, so that by applying one hand to the outside tube, and the other hand to the end of the smallest tube, the telescope may at one pull be drawn out to its full length; then any of the tubes (that next the eye is most generally used) may be pushed in gradually, while you are looking through it, till the object is rendered distinct to the eye. To make the tubes slide properly, they all pass through short springs or tubes; these springs may be unscrewed from the ends of the sliding tubes, by means of the milled edges which project above the tubes, and the tubes taken from each other if required, and the springs set closer if at any time they be too weak.

Fig. 5 represents the exterior tube of the telescope, which is to be unscrewed from the rest, at m l, as it does not make any part of the microscope; the cover, k, which protects the object-glass, serves also as a box to contain two ivory wheels, Fig. 1 and 2, with the objects, and a small mirror, Fig. 6.

Fig. 4 is a view of this cover when taken off: unscrew the top part of it, and the mirror, Fig. 6, may be taken out; unscrew the cover of the lower part, and you will find therein the two circular object-wheels above mentioned.

Fig. 3 represents the three internal tubes of the telescope, which constitute the microscopic part thereof. Draw the tubes out in the manner as shewn in the figure; then at the inside, but at the lower end of the exterior tube, a, you will find a short tube, which serves as a stage to hold the object and support the mirror; pull this tube partly out, and turn it, so that a circular hole which is pierced in it may coincide with a similar hole in the exterior tube. This tube is represented as drawn out at Fig. 3, the mirror, Fig. 6, placed therein at b c, and the transparent object-wheel fixed at a.

Fig. 1 represents the slider with transparent objects.

Fig. 2, that with the opake. They are made of ivory, and turn on a pin at the center; the slit end of this pin fits on the edge of the tube, which is then to be pushed up, so that the lower end of the exterior tube may bear lightly on the upper side of the slider, agreeable to the view which is given at a, Fig. 3. Now push down the second tube till the milled part falls on the milled edge of the extreme tube, being careful of the circular hole in the exterior one. Nothing now remains to be done but to adjust for the focus, which is effected by pushing in the tube R, and moving only the first, n.

The instrument may be used in two ways for transparent objects: first, in a vertical position, when the light is to be thrown on the object by the mirror, b c; or it may be examined by looking up directly at the light; in the latter case the mirror must be taken away. In viewing opake objects the mirror is not used; as much light as possible must be admitted on them through the circular holes of the tubes. Any object may be viewed by first pushing in the tube, R, and then bringing the tube, n, to its focal distance from the object. The telescope, when shut up, is about eight inches in length, and when drawn out, is about twenty inches. It is of the achromatic construction.

DESCRIPTION OF AN INSTRUMENT FOR CUTTING THIN TRANSVERSE SECTIONS OF WOOD, Plate IX. Fig. 1.

It consists of a wooden base, which supports four brass pillars; on the top of the pillars is placed a flat piece of brass, near the middle of which there is a triangular hole. A sharp knife which moves in a diagonal direction, is fixed on the upper side of the afore-mentioned plate, and in such a manner, that the edge always coincides with the surface thereof. The knife is moved backwards and forwards by means of the handle, a. The piece of wood is placed in the triangular trough, which is under the brass plate, and is to be kept steady therein by a milled screw which is fitted to the trough; the wood is to be pressed forward for cutting, by the micrometer screw, b. The pieces of wood should be applied to this instrument immediately on being taken out of the ground, or else they should be soaked for some time in water, to soften them, so that they may not hurt the edge of the knife. When the edge of the knife is brought in contact with the piece of wood, a small quantity of spirit of wine should be poured on the surface of the wood, to prevent its curling up; it will also make it adhere to the knife, from which it may be removed by pressing a piece of blotting paper on it.

Fig. 2, is an appendage to the cutting engine, which may be used instead of the micrometer screw, being by some practitioners preferred to it. It is placed over the triangular hole, and kept flat down upon the surface of the brass plate, while the piece of wood is pressed against a circular piece of brass which is on the under side of it. This circular piece of brass is fixed to a screw, by which its distance from the flat plate on which the knife moves may be regulated.[38]

[38] Many other kinds of cutting engines have been constructed, but the specimens from them have not yet appeared with that perfection which is requisite to this sort of objects; whether it lies in the preparation of the woods, or engine, I do not take upon me to determine. Mr. Custance has certainly produced the most exquisite. Edit.


                                                                                                                                                                                                                                                                                                           

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